Investigations On The Immunological Basis Of Diagnosis And Therapeutics For Leukemia

With development of immunology and elucidation of contribution of immunological mechanism in pathogenesis of leukemia, immunological technologies, methods and principles play more and more important roles in diagnosis and therapeutics for leukemia. In the present study, we investigated the anti-leukemia activity of a novel vaccine and the function of novel interleukins in hematopoietic system, and made several progress.Part Ⅰ: Construction of murine β-defensin 2 tumor vaccine and its antileukemia activity in murine L1210 modelFor many years, innate immunity has been regarded as to be of secondary importance in the hierarchy of immune functions. However, it has become increasingly clear that the innate immune system is essential for the activation of the adaptive immune response and its direction into a particular effector type, and allows the adaptive immune response to discriminate between self and nonself. Murine p-defensin 2 (MBD2) is a small antimicrobial peptide of the innate immune system. Recent study demonstrated that MBD2 could not only recruit immature dendritic cells, but also activate them by Toll-like receptor 4, inducing up-regulation of proinflammatory factors, chemokines and costimulatory molecules. Thus it may provide a critical link between the innate immune system and the adaptive immune response. In this report we examined the antileukemia activity of MBD2 in a murine model of acute lymphocytic leukemia (ALL) L1210. Gene for mature MBD2 was cloned from LPS-treated DBA/2 mice skin by RT-PCR. The cDNA encoding the mature MBD2 was fused with a mouse Igκ signal sequence by overlap PCR amplification. The construct was verified by the DNA sequencing. The MBD2-expression plasmid or empty plasmid vector was transduced into L1210...