The Pharmacokinetic Study Of Chinese Traditional Medicine Of Pogostemon Cablin (Blanco) Benth And Picrorhiza Kurroa Royle Ex Benth

Patchouli alcohol and patchouli oil extracted from Pogostemon cablin (Blanco) Benth. and picroside II and kurroa glucosides extracted from Picrorhiza kurroa Royle ex Benth. were used for pharmacokinetics study.A capillary gas chromatographic method was developed for the pharmacokinetic study of patchouli alcohol in the plasma, tissues, urine and dejection of rats. The patchouli alcohol in samples was extracted with ethyl acetate and eugenol was used as internal standard. The separation was carried out on a HP-5MS fused silica capillary column with flame ionization detector (FID) and high-purity nitrogen as carrier gas, utilizing programmed oven temperature. The linearity and repeatability of this method were satisfactory and its recovery was from 90% to 110% with relative standard deviation (RSD) less than 10%.The sensitive and simple high performance liquid chromatography method with UV detection was developed and validated for determining picroside II in the plasma, tissues, urine and dejection of rats. The sample pre-treatment procedure consists of deproteinization by addition of acetonitrile. The analysis was done on a Shimadzu VP-ODS column (250 mm × 4.6 mm i. d., 5 μm). The mobile phase consisted of acetonitrile and 0.1% acetic acid aqueous (v/v), at a rate of 1 mL/min. The linearity, repeatability and recovery of this method were satisfactory for determination picroside II.Patchouli alcohol showed linear pharmacokinetics in rats, rabbits and dogs, and the concentration-time data were consistent with the two-compartment open model after patchouli alcohol or patchouli oil i.v. administrated. The t_1/2β of patchouli alcohol was less than that of patchouli alcohol in patchouli oil, and this suggested the elimination of patchouli alcohol in patchouli oil was affected by the other compounds.The bioavilability of patchouli alcohol or patchouli alcohol in patchouli oil was less than 40% and patchouli alcohol in patchouli oil was much less than that of patchouli alcohol, and this suggested the absorption of patchouli alcohol in patchouli oil was affected by the other compounds.Patchouli alcohol or patchouli alcohol in patchouli oil was mainly distributed in intestine, stomach, liver, spleen and kidney and it was also found in other tissues after i.v. administrated to...