Studies On The Chemical Constituents Of Semiaquilegia Adoxoides And Trollius Ledebouri

In this doctoral dissertation, the phytochemical and pharmacological studies were carried out on the medicinal plants of the family Ranunculaceae, Semiaquilegia adoxoides (DC.) Makino and Trollius ledebouri Reichb.. Before the research working, the advances in the recent research of chemical constituents and pharmacological activities of Trib. Isopyreae plants were reviewed.Ten compounds (S1-S10) were isolated from the ethanol extract of the roots of Semiaquilegia adoxoides by using the advanced preparative chromatography techniques. And on the basis of the spectroscopic analysis and chemical methods, their structures were determined as follows: triacontane (S1), lacceroic acid (S2), β -sitosterol (S3), palmitic acid (S4), aurantiamide acetate (S5), daucosterol (S6), cirsiumaldehyde (S7), griffonilide (S8), butyl-β-D-mannopyranoside (S9), and lithospermoside (S10). All the compounds except for S3, S8 and S10 were isolated from the genus Semiaquilegia for the first time. Among of them, the pharmacological activities of the compounds S8, S9 and S10 were tested on high throughput screening. The results showed that only the compounds S8 and S9 could strongly inhibit AchE activity. Thus, the research results will provide a scientific basis for deep probe into the fields concerning researching and developing new active compounds.Twenty-nine compounds were obtained and identified from the ethanol extract of the flowers of Trollius ledebouri. On the basis of the modern spectroscopic analysis (IR, UV, MS, ¹H NMR, ¹³C NMR, HMQC, HMBC and NOESY), as well as classical chemical methods, eleven new copounds of them were determined. Among the new compounds, there...