Study On Quality Standard And Phamacological Action From Trollius Ledebouri Reicrb. (Chinese Medicinal Herbs)

Ranunculaceae (Trollius) are around 31 kinds of plants,16 species in our country. Wherein Trollius chinensis Bung has been widely used in folk, its flowers on behalf of the tea available to treat respiratory tract infections, tonsillitis, pharyngitis, acute otitis media and other diseases,and the signifi-cant effect that has been Included in the " Great Dictionary of Chinese Medicine " and believe flavonoids are the main active ingredient Trollius. Currently prescribed preparation and compound preparation of Trollius chinensis are used in clinical treatment, including tablets, granules, oral and teabag etc.Trollius ledebouri Reichb as congener of Trollius chinensis,in the north-east, is widely daxing’an mountains, xiaoxing’an mountains, zhangguangcai mountains and Flows of Changbai Mountains, is rich in resources, but has not yet been fully exploited. As compared with Trollius chinensi sthere’s an study on quality standard and pharmacological action from Trollius ledebouri to explore the feasibilityTrollius ledebouri is used in medicine.in this articleBy Pharmacognosy studies to determine the features and identification in microstructure between Trollius chinensis and Trollius ledebouri; and by thin layer chromatography and UV spectrophotometry make quantitative analysis about; flavonoids from Trollius chinensis and Trollius ledebouri; results show similar chemical composition and total flavonoid content is also quite. HPLC method was used to compare fingerprints, which share peaks 21 and 22, respectively. The total flavonoid content as an indicator to determine the optimal harvest of Trollius ledebouri Reichb; the results showed a higher yield of herbs and total flavonoid content in mid-June to the end. Using orthogonal design method to optimize the extraction process and purification process of total flavonoids Trollius ledebouri Reichb, the result is 65% ethanol, extracted three times, each time 2h, each 10 times the amount of ethanol; and the content of total flavonoids refined, more than 60%, to conform to the requirements of Chinese medicine formulations Class V new drugs. The result provides a reference on the control valve of medicinal quality about Trollius ledebouri ReichbEarly pharmacodynamic studies have shown that Trollius ledebouri Reichb has antibacterial effect, anti-inflammatory analgesic, anti-oxidation. Studies of Trollius ledebouri Reichb extract on fever, cough and phlegm in vitro antiviral and enhance immune effects in this article, the results showed that Trollius ledebouri Reichb extract has inhibition to respiratory syncytial virus, influenza virus type A 3 and the growth of COX-B; rabbits fever and endotoxin-induced rat fever caused by yeast are obvious antipyretic effect; ammonia induced cough was inhibited in mice and increased tracheal phenol red excretion; increase the mouse mononuclear phagocyte function and serum hemolytic serum antibodies generated in mice, and significantly promote the mouse delayed type hypersensitivity. Tip is that Trollius ledebouri Reichb extract has enhanced immune function.To sum up the test results indicate that:Trollius ledebouri Reichb is approximate chemical composition and total flavonoid content also with Trollius chinensis, its pharmacological effects show good antibacterial and antiviral activity, it can be a substitute resources of traditional Chinese medicine Trollius chinensis.