Induced Drug Resistance And Explored The Changes Of The Activity Of Thioredoxin Reductase In Pancreatic Cancer Cell Line

Background: Carcinoma of the pancreas is a disorder of high malignance, poor resectability and easy recurrence after operation, whose incidence has yet increased gradually in recent years. Chemotherapy has played a great part in the integrated therapeutic system of pancreatic cancer. Unfortunately, however, most pancreatic cancers show no sensitivity to chemotherapy and some still others rapidly turn resistant after several doses. The clinical application of Gemcitabine in the past years has eventually shed a light on the efficacy of chemotherapy, but in general the integral effect of chemotherapy is not far from satisfactory. It is thus of great importance to explore the resistance mechanism of pancreatic cancer. The laboratory has conducted quite many studies of the mechanisms of primary and secondary drug resistance, and successfully induced the relevant human drug resistant cell strains of pancreatic cancer with 5-fluorouracil (5-Fu) and Adriamycin (ADM) respectively. In this study, a first line medication, Gemcitabine, which is currently applied in the treatment of pancreatic cancer, was selected to induce the pancreatic cancer cell...