Effects Of Qidan Granule On The Expression Of Ⅰ Procollagen And Ⅲ Procollagen MRNA In Pulmonary Fibrosis Rats Induced By Bleomycin

Objective: To observe the effects of Qidan granule on the mRNA expression of type I procollagen and type III procollagen in pulmonary fibrosis rats induced by bleomycin by in situ hybridization and to explore its pathogenesis.Methods: 160 SD rats in good conditions were divided into six groups at random: normal control group(N), model group(M), Qidan granule group 1 and 2(Q1,Q2) and Hydrocortisoni group land 2(H1,H2). Normal group was treated with saline water. Pulmonary fibrosis model was induced by intratracheal instillation of bleomycin (5mg/kg body weight). 40 rats used as model group and other rats were treated with Qidan granules(3125mg/kg/d) or treated with Hydrocortison.(25mg/kg/d) respectively. Rats were sacrificed at 7, 14, 28 or 42 day post-Bleomycin and the lungs were harvested and fixed in paraformaldehyde and embedded in paraffin wax for histopathological examination and detection of expression of type I procollagen and type III procollagen mRNA by in situ hybridization. All the results were analyzed by Leica Qwin V3 digital-image processing system.Results: Qidan granule could obviously reduce alveolitis and fibrosis. III procollagen mRNA expression was increased in the acute alveolitis phrase of pulmonary fibrosis induced by bleomycin, the expression of type I procollagen mRNA was mainly increased in the chronic stage of pulmonary fibrosis. The expression of III procollagen mRNA in Qidan granule group was highest at 14 day...