In Vitro Induction Of Anti-tumor CTL Effect Against Hematologic Malignancies By Dendritic Cells Infected With Recombinant Adenovirus Vectors Containing Human Survivin And GM-CSF Genes

Relapse after chemotherapy could lead to poor prognosis in patients with leukemia or lymphoma. By using tumor-specific immunotherapy to eradicate minimal residual diseases (MRD) could prevent disease relapse and improve outcome of these patients. Our study aims at exploring whether peripheral blood derived dendritic cells (DC) expressing human GM-CSF and/ or survivin can enhance their capability to induce human T lymphocytes into tumor-specific cytotoxic T lymphocytes (CTL), which might provide preliminary laboratory evidence for the application of these modified peripheral blood mononuclear cells(PBMNC) in tumor-specific immunotherapy.ObjectivesBy constructing recombinant adenovirus vectors containing human survivin and GM-CSF genes, and generating peripheral blood mononuclear cells-derived DC, the capability of DCs transfected with recombined adenovirus were tested in inducing cytotoxic T cell effect against human leukemic cells. Therefore the sdudy provides laboratory evidence for the application of tumor-specific immunotherapy.