The Effect On Tumor Cells And Subsequent Developmen After Intrauterine Transplanted Into The Mouse Fetus

Background Successful intrauterine transplantation (IUT) of stem cells for fetal defects in some animal models and human diseases has prompted us to study the mechanisms of the transplantation, immunologic tolerance and embryonic environment. However, the embryonic development and IUT remain to be understood. The objective of this study was to determine whether intrauterine transplantation of tumor cells would affect the survival and growth of the tumor cells themselves as well as the fetus development. Methods NIH inbred mouse embryos of D9^D12 and D13^D18 were transplanted with 6×10⁶ H₂₂ cells and S180 cells into amniotic cavity and abdominal cavity, respectively, through the uterus of the pregnant mothers. The adult NIH mice and newborn NIH mice which were inoculated the same number of H₂₂ cells and S180 cells by intraperitoneal injection were used as positive controls for the cancer bearing control group. Undisrupted fetuses of the same gestation were used as negative controls (i.e., for the normal development) group. The tumor growth and development of fetuses transplanted with tumor cells in uterus...