The Expression Of HMGB1 And The Depressant Effect Of Ethyl Pyruvate In Endotoxin-Stimulated Macrophages

objective:Sepsis is a lethal systemic inflammatory reaction caused by bacterial infection. It is one main causes of death in clinical severe patients. The basic reason of sepsis happened and developed is the excessive delivery of cytokines and inflammatory mediators by organism, which leads to the inflammatory reaction loss of control and immune function disorder. Therefore, the cytokines and inflammatory mediators play an important role in the development of sepsis. High mobility group protein box1 (HMGB1) is a kind of advanced stage inflammatory mediators discovered recently. It is the critical inflammatory mediators in generation and development of sepsis. And more and more people pay close attention to it's role in sepsis. But the molecule mechanisms of HMGB1 release in sepsis are still unknown. Firstly, we used endotoxin (lipopolysaccharide,LPS) to stimulate macrophages and observed the releasing and expressing level of HMGB1, tumor necrosis factor alpha (TNF-α) and Interleukin-6 (IL-6).Then we determined the activity changes of Nuclear factor kappa B (NF-κB)﹑P38 mitogen activated protein kinase (p38MAPK) and CREB binding protein (CBP), in order to elucidate the release rule and molecule mechanisms of HMGB1 after stimulated by LPS. Secondly, we detected the releasing and expressing level of HMGB1, TNF-αand IL-6 in macrophages stimulated by LPS﹑LPS+ethyl pyruvate(EP) respectively...