Study Of Relationship Between Acetylcholine Receptor Antibody Purified From Myasthenia Gravis Sera And Neuronal Nicotinic Acetylcholine Receptor Of The Central Nervous System In The Rat

OBJECTIVE Immunoreaction between acetylcholine receptor antibody (AChRab) from myasthenia gravis(MG) patients and neuronal nicotinic acetylcholine receptor (AChR) and the action of AChRab on rat brain stem auditory transmission were investigated by immunocytochemistry, brain stem auditory evoked potential (BAEP) and Western Blot analysis in search for the mechanism of central nervous system (CNS) involvement in this disorder. METHODS. SD rat brain sections were incubated with AChRab and IgG fractions from normal healthy control sera to clarify whether AChRab present in MG sera and cerebrospinal fluid could bind to neuronal nicotinic AChR. To explore specific binding of AChRab to neuronal nicotinic acetylcholine receptor, rat brain sections were preincubated with...