Cloning Of The The V_H/V_L Of The 9.1C3 McAb And Construction And Expression Of 9.1C3 ScFv

It was found that NK cell plays an important role in the development of some auto-immune diseases and graft rejection ,especially in the early stage of graft-versus-host reaction after bone marrow transplantation. 9.1C3 molecule is a kind of glycoprotein expressed on peripheral blood mononuclear cells .Previous studies showed that 9.1C3 McAb could markedly block the cytotoxic effects mediated by NK and LAK cells etc., however, it could not affect the binding of effectors to the targets, indicating that 9.1C3 molecules may play an important role in the late stage of the killing course mediated by the killer cells . Hence, 9.1C3 McAb not only displays a potential application value in treating some auto-immune diseases, but also possesses an important theory value in exploring the mechanisms of the cytotoxicity mediated by NK cells. Because the mouse-derived parental antibody possesses antigenicity...