An Experimental Study Of Biological Effects Of Platelet-Derived Growth Factor On Brain Injury

Platelet-derived growth factor(PDGF) is a polypeptide with multiple biological effects and a potent mitogen and chemical inducer in vivo, it widely distributed in platelets, mononucleur phagocyte system, fibroblast neuron and gliocyte. It' s important in the growth and development of tissues, wound healing, and the formation and development of athrosclerosis (As) and tumors. Resent studies showed that PDGF-A chain, PDGF-B chain and their receptors are widely presented in the central nervous system, and their expression is enhanced after brain injury. This study proved that PDGF plays important roles not only in neurite sprout and remyelin of injuried neuron, also in microvascullar regeneration of CNS in injuried area. The present study has been designed to investigate the effects of PDGF on the brain injury and to make a better understanding of the mechanism of protecting action of PDGF in the CNS during injury and healing process.Main Procedures:1. Highly purified portine platelet-derived growth factor (pPDGF) was prepared for experimental from portine platelets by using serial methods of acid-etha-...