The Preliminary Study On Detection Of HSP70 In Serum Of Patients Infected With HCV And Inducing HCV CTLs In Vitro Utilizing HSP-peptides Complex

Hepatitis C virus(HCV) is a major etiologic agent of acute and chronic hepatitis, cirrhosis and hepatocellular carcinoma in the world. The host humoral immune response fails to clear viral variants because of the high rate of genetic variability of HCV.Available evidence suggested that there were many cytotoxic T lymphocyte cell (CTL) epitopes in HCV proteins. Especially C protein can cause lymphocyte cell proliferation and induce CTL that can restrain growth of HCV. These facts implicated that cellular immune response may play an important role in controlling of HCV infection. Because induced CTLs are faint generally, it is important to strengthen them. Many studies showed that heat shock protein (HSP), as a molecular chaperone, played important roles in the processing and presentation of MHC-I antigens of virus or tumors. HSP can assist binding peptides to present epitope with MHC molecule and induce Ag-specific CTLs. The aim of our study is to investigate the expression of HSP70 and HSP70 antibody in serum of patients infected with HCV and the role of HSP-peptide complex in induction of specific CTLs in vitro. This may provide some fundamental data on new HCV vaccine and effective tools for controlling of HCV. Our study include 4 parts as follow: 1. Detection of HSP70 in peripheral blood110 serum samples from HCV patients, HBV patients and normal individuals were detected by enzyme-linked immunosorbent assay (ELISA)...