| Objective: In tumor cells, the chaperoning activity of HSP90α is subverted to retain the functions of mutant proteins and thus facilitate the malignant transformation.Meanwhile, HSP90α can also be secreted by tumor cells.HSP90α can promote tumor invasiveness and is highly correlated with tumor malignancy, especially tumor metastasis. In this review, we discuss the known molecular mechanism of HSP90α and its involvement in cancer and in particular, oesophageal carcinoma.The aim of this study was to be measureed the expression of Secreted Heat Shock Protein 90 α in Patients with Esophageal Squamous Cell Carcinoma before and after radiotherapy.This study was performed to detect secreted HSP90 alpha expression in esophageal squamous cell carcinoma before and after radiotherapy, which aimed to explore the relationshipbetween secreted HSP90 alpha and the clinicopathological characteristics including gender, age, clinical stage, tumor size, differentiation,and analyse the clinical significance of secreted HSP90α with radiotherapy.Methods:1.Collected 64 cases previously untreated patients of esophageal squamous cell carcinomafrom December 2013 to July 2014 in our hospital and another 20 cases of normal people as control group.2.Using enzyme-linked immunosorbent assay(ELISA) for quantitative detection of secreted Hsp90αin esophageal squamous cell carcinoma beforer and after radiotherapy.3. Statistical analysis: The data was analyzed by SPSS 17.0, using nonparametric(the Mann- Whitney) test, analysis of variance(ANOVA),A probability(P)-value less than0.05 was considered statistically significant.Using the receiver operating characteristic curve(ROC) to determine the cut-off point.Results:1.Secreted HSP90α level in esophageal squamous cell carcinoma(37.3656 ±28.1257, n = 64,0 ~ 144.3) was significantly higher than the control group(9.8413 ±17.4857, n = 20,0 ~ 67.43, P = 0.000), with statistical significance.2. At the cut-off point 8.7125 on the receiver operating characteristic curve,secreted HSP90α could comparatively discriminate esophageal squamous cell carcinoma from healthy control groups with sensitivity of 0.923,specificity 0.750.3. Secreted HSP90α have no relation with gender(F= 0.837, P = 0.364),age(F = 0.738,P = 0.394) in esophageal squamous cell carcinoma. It was related with tumor diameter(F = 5.091, P = 0.018),clinical stage(F = 3.42, P = 0.024),histological grade(F = 7.59,P = 0.007) and lymph node metastasis(F = 2.58, P =0.032),with statistical significance.4. The expression of secreted HSP90α in esophageal squamous cell carcinoma before and after radiotherapy( X ±s: 31.36 ± 34.28, 33.94 ± 19.78, 2.59 ± 38.16) have no statistical significance(t=0.334, P=0.743).Conclusion:1.Secreted HSP90α level in esophageal squamous cell carcinoma was significantly higher than the control group.High sensitivity and specificity suggest that secreted HSP90 alpha may be potentially useful tumor markers in esophageal squamous cell carcinoma, with higher application value in the diagnosis, treatment and prognosis.2. Secreted HSP90α have no relation with gender and age in esophageal squamous cell carcinoma.There was a correlation between tumor diameter, the clinical stage,histological grade, lymph node metastasis,suggesting that Secretory HSP90α play a certain role in occurrence, development of esophageal carcinoma.3. The expression of secreted HSP90α in esophageal squamous cell carcinoma before and after radiotherapy have no statistical significance, suggesting that radiotherapy may have no effect on the expression of HSP90 alpha in plasma,and Secreted HSP90α may not be used as evaluation index for the effect of radiotherapy. |
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