The Study And Application Of The Monoclonal Antibodies Against Haemorrhagic Fever With Renal Syndrome Virus

1. Thirty-six strains of HFRS virus isolated from patients and a numler of host animals in various areas in China were analysed by IFAT using 10 McAbs against HFRS virus. Antigenic differences among the strains have been demonstrated. The HFRS virus strains revealed nine different reactions with the McAbs, showing that there are at least nine different antigenic determinants including group, type- and strain-specific. Analysis of the results shows that antigenic differences among the HFRS virus strains are Mainly related to differences in the host animals and may be one of the causes resulting in the variations in severity of the HFRS and the different clinical and epi-demiological characteristics.2. An affinity chromatography method has been developed to purify HFRS virus structural protein. HFRS virus Has first purified roughly by protamine sulfate and polyethylene glycol precipitation from brain...