An Experimental Study On The Oral Administration Of Insulin And The Mechanisms Of Its Absorption By The Gastrointestinal Tract

background Oral administration of insulin would be particularly convenient for diabetic patients. Since insulin is a peptide, for its oral administration, insulin has to be associated to a drug carrier that prevents proteolytic degradation of the peptide in the gastrointestinal tract and transported from the gut lumen to the blood compartment. Polyalkyocyanoacrylate nanoparticles are spherecal vesicals, both biocompatible and biodegradable . Some reports suggested that they can protect the encapsulated polypeptide from proteolysis and pass through the intestinal epithelium .Objective The aim of this study is to investigate the hypoglycemic effect of INP prepared by improved emulsion polymerization method and the factors affecting the INP size and loading efficiency in order to prepare smaller INP easily absorbed by gut and choose the better oral dosage formulation in the presence of protease inhibitor and absorption enhancer for INP. The mechanism of the gastrointestinal absorption of the oral insulin, the distribution of INP and the effect of the complex oral insulin on the intestinal epithelum are also to be investigatedMethods and Results.1. INP parepared with improved emulsion polymerization method are 35nm in diameter with insulin entrapment efficiency 95% by HPLC.