| Chapter 1 Study on the 1st, 2nd and 3rd trimester placental factorsagainst HIV-1 in vitroObjective: To investigate the effects of the 1st, 2nd and 3rd trimester placental factors (PFs) on human immunodeficiency virus type 1 (HIV-1) infection in vitro, and to evaluate their possible roles in HIV-1 intrauterine infection.Methods: Calcein-AM stained H9/HIV-1â…¢B cells were treated with different concentrations of the 1st, 2nd and 3rd trimester PFs respectively, then mixed with MT2 cells, HIV-1-mediated syncytium formation was observed under fluorescent microscopy. MT2 cells were treated with cell-free HIV-1 â…¢B, then washed and cultured with different concentrations of the 1st, 2nd and 3rd trimester PFs respectively, the protecting effect of PFs on HIV-1-infected cells was determined with MTT, viral replication was evaluated by measuring the levels of p24 antigen in culture supernatants with ELISA.Result: PFs did not suppress HIV-1-mediated syncytium formation, but they significantly increased HIV-1-infected cells' viability and decreased p24 antigen levels. Moreover, their effects were trimester- and concentration-dependent.
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