| Part One:Study on the relationship between HBeAg of fetus and Intrauterine vertical transmission of hepatitis B virusAbstract:Background and Objective HBeAg of the pregnant women and neonates is a high risk factor of Intrauterine vertical transmission of HBV,but it is unknown that if HBeAg is the direct reason of intrauterine infection of HBV.Our research is to study the mechanism of maternal-infant vertical transmission of hepatitis B virus by testing the influence of the HBeAg on the immunologic state of neonates.Methods 50 neonates born from positive HBsAg mother were divided into positive HBeAg group(n=23)and negative HBeAg group(n=27).By using diantibody sandwich enzyme linked immunosorbant assay(DAS-ELISA)and polymerase chain reaction(PCR),the serum HBV M and HBV DNA were detected in these pregnant women and neonates,IFN-γ,IL-2,IL-4 and IL-6 in these neonates were also detected by using DAS-ELISA,and theses cytokine levels were compared between the two groups,the control group(20 neonates born from negative HBsAg mothers)was set meanwhile.Results Pregnant women were divided into HBeAg group(27 cases)and negative HBeAg group(23 cases),the cases of positive HBeAg neonates of each group were 22(81.48%)and 1(4.35%),The number of positive HBeAg neonates born from positive HBeAg mothers was much more than it is of the positive HBeAg neonates born from negative HBeAg mothers,which had significant difference(P＜0.05);The IL-2,IFN-γlevels of the positive HBeAg group neonates were significant lower than those of the negitive HBeAg group(26.08±5.12pg/ml vs 32.16±6.26pg/ml,12.14±1.89pg/ml vs 17.20±5.39pg/ml),the IL-4,IL-6 levels were signifinant higher 17.35±3.18pg/ml vs 11.03±2.64pg/ml,24.01±4.12pg/ml vs 17.91±2.81pg/ml),and those of the control group were(33.39±9.69pg/ml,19.27±3.80pg/ml, 12.77±4.29pg/ml,18.35±3.24pg/ml),which had no significant difference with the nagative HBeAg group(P＞0.05).Conclusion The HBeAg of positive HBsAg pregnant woman can be transmitted into her neonates and causes immunity unbalance,which makes the organism can not clear the HBV in time and efficiently and brings on the immunotolerance of hepatitis B virus meanwhile,and these mechanisums above-mentioned have the close relationship with HBV maternal-infant vertical transmission. Part two:study on the distribution in human placentaAbstract:Background and Objective The placental tissue infection is a high risk factor of the occurrence of HBV intrauterine vertical transmission,the former researches show that the HBV in the placenta exist by means of HBsAg-anti-HBs-C3c complex,the later can attached to the cytoplasm of trophobasts and combined with the related acceptor of Ig-G or C3,which has the important significance of neonates' intrauterine infection;some studies discovered that FcγRⅢexisted in the placenta,which might be very important to the HBV intrauterine transmission,but there is no research to show that if there is acceptor of C3c in the placenta.The acceptor of C3 is CR1(CD35).Our experiment is to study the research of the HBV placenta infection by testing the expression of CD35 on the placenta.Methods 6 negative pregnant women and 3 normal women were studied in our study,placenta of which were detected by immunohistochemistry SABC to show the situation of CD35expression on placenta.Results the result showed that CD35 expressed on the blood cells of placenta blood vessels,but there was no CD35 discovered in placenta trophoblastic cells and interstitial cells.Conclusion CD35 does not exist in placenta and can not cause the HBV intrauterine infection by combining with the HBsAg-anti-HBsAg-C3c complex.
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