Study Of Apomorphine Induced Abnormal Involuntary Movement And Cell Therapy On Parkinson Rat

Objective Observed the abnormal involuntary movement of Parkinson rat model induced by Apomorphine. Use animal Positron Emission Tomography to observe the dopamine system injury and the effect of apomorphine on Parkinson rat brain, and observed the cell therapy on the rats with dyskinesia. Methods We gave two 6-hydoxydopamine injection into right medial forebrain bundle (MFB) to setup completely unilaterally Parkinson rat model and observed their abnormal behavioral under both free and apomorphine used situation. According AIM scores, the rat divided into two groups: high dyskinesia and low dyskinesia groups. Both were transplanted with retinal pigment epithelium cell.. use 2-carbomethoxy-3h-(4-fluorophenyl) tropane (¹¹C-β-CFT) and ¹⁸FDG animal PET to image change of dopamine system and the glucose metabolism on the different regions of brain under the both situation. and observed function of the graft in the host brain.Results: The animal model showed the lose of that initiation of stepping movements by the left(contra-lateral)paw were greatly reduced, and initial time prolonged compared to the control side. The ¹¹C-CFT image showed that the depletion of presynaptic maker dopamine transporter(DAT) in the lesion side's striatum, MFB and substantia nigra. Although the function of left paw stepping movements, initial time partly improved on the apomorphine used situation, the model showed the contraversive rotation and lesion side abnormal involuntary movement, such as limb dyskinesia, axial dystonia and masticatory dyskinesia, and FDG image showed the focal increased glucose metabolism in the lesion side (part of cortex, part of striatum and thalamus) which glucose were reduced under free situation. Lateral spetal of the lesioned side that metabolism was higher under free situation were reducing on the Apomorphine situation; but the glucose metabolism of Globus pallidus get a further increase.. Conclusion Completely unilaterally Parkinson rat model showed extensive neuropathology and profound loss of sensorimotor on the lesioned side. For the loss of adjust function of the system, dopamine agonist only partly improved thefunction of Parkinson rat's injury side, and induce the AIM. The PET can view thefunction of the brain on a big picture.