Research To The Mechanism Of The Function Of Nerve Rotection To The Secondary Injury In Intercerebral Hemorrage By Acupuncturing Scalp Cave On The Experiment Rat

Objective: Research to the mechanism of the function of nerve protection to the secondary injury in intercerebral hemorrage by acupuncturing scalp cave on the experiment rat .Methods: Selected the healthy Wistar male rats of 160 by examination.The rats were assigned into 3 sets randomly: model set, pinprick set, piracetam set.containing 50 rats in each.Each set were again divided into five time phase point of 6 hours, 1 day, 2days, 3days, 7days .containing 10 rats in each. All rats were Made to be the intercerebral hemorrage model. Another 10 rats were assigned as the matched blank set. The pinprick set were acupunctured on the "Baihui" penetrating"Qubin".We Observated the damage extent of the nerve function of each set in deferent time phase point with the neuroethology index; Observated the change of the brain histomophology of each set in deferent time phase point with light microscope and electron microscope; Observated the change surrounding the hamatoma of the messengerRNA and the proteinum expression of NGF, IL-1βof each set in deferent time phase point with the methods of immunity histochemistry and in situ hybridization ; Observated the influence of the nerve apoptotic of each set in deferent time phase point with the method of Terminal-deoxynedeotidyl transferase mediated d-UTP nick end labeling.Results:1.The model set had appeared different level nerve function defection and damage performance. The 3 days set's nerve function defection and damage was most serious. Compare with the model set, the pinprick set's nerve function defection and damage was abatement in the same time phase point. Both were relatively showed the significant difference (p<0.01) and havd the statistical significant. Compare with the piracetam set, the pinprick set's nerve function defection and damage was relatively showed no significant difference in deferent time phase point (p>0.05) and had no statistical significant.2.It was discovered that the model set's brain tissue surrounding the hematoma was necrosis, dropsy, karyopyknosis of never cells, vacuolization and phlegmasia cells infiltrated with the method of observation under light microscope. The 3 days set was most serious.Compare with the model set, the pinprick set's damage was abatement in deferent time phase point. Compare with the model set, piracetam set's damage was relatively showed no significant difference in deferent time phase point .3. It was discovered that the model set's brain tissue surrounding the hematoma was swelling of the nerve cellular, chromatin agglutination, nucelus dissolve, bioblast vacuolization, cristae split, rough endoplasmic reticulum dilatation, polysome depolymerize and synaptic bouton engorgement.Compare with the model set, the pinprick set's damage was abatement in deferent time phase point. Compare with the model set, piracetam set's damage was relatively showed no significant difference in deferent time phase point .4.The detecting result of IL-1βwith the methods of immunity histochemistry and in situ hybridization: In the model set and the pinprick set , the expression of IL-1βof rat's brain tissue surrounding the hematoma was increased gradually and to the peak in 3days with the increasing of different time phase point , but decreased gradually in 7days.In the same time phase point, Compare with the model set, the pinprick set's expression of IL-1βwas lower obviously. Both were relatively showed the significant difference (p<0.01) and havd the statistical significant. In the same time phase point, Compare with the piracetam set, the pinprick set's expression of IL-1βwas lower obviously .Both were relatively showed the significant difference ( p<0.01) and havd the statistical significant.5.In the model set and the pinprick set, the number of nerve apoptosis of the rat's brain tissue surrounding the hematoma was increased gradually with the increasing of deferent time phase point and to the peak in 3days, but decreased gradually in 7days With the examination of TUNEL method. In the same time phase point, Compare with the model set, the pinprick set's number of apoptosis nerve cell was lower obviously .Both were relatively showed the significant difference (p<0.01) and havd the statistical significant. In the same time phase point, Compare with the piracetam set, the pinprick set's number of apoptosis nerve cell was lower obviously .Both were relatively showed the significant difference (p<0.01) and havd the statistical significant.6. The detecting result of NGF with the methods of immunity histochemistry and in situ hybridization: In the pinprick set, the expression of NGF of rat's brain tissue surrounding the hematoma was increased gradually and to the peak in 7days with the increasing of deferent time phase point .In the same time phase point, Compare with the model set, the pinprick set's expression of NGF was higher obviously .Both were relatively showed the significant difference (p<0.01) and havd the statistical significant. In the same time phase point, Compare with the piracetam set, the pinprick set's expression of NGF was higher obviously .Both were relatively showed the significant difference (p<0.01) and havd the statistical significant.Conclusions: 1. Acupancturing scalp cave on the rat of the experiment intercerebral hemorrage can relieve the extent of the neurologic impairment.2. Acupancturing scalp cave on the rat of the experiment intercerebral hemorrage inhibit the expression of IL-1βof rat's brain tissue surrounding the hematoma.3. Acupancturing scalp cave on the rat of the experiment intercerebral hemorrage can inhibit the nerve apoptosis of the rat's brain tissue surrounding the hematoma4. Acupancturing scalp cave on the rat of the experiment intercerebral hemorrage can promote the expression of NGF of rat's brain tissue surrounding the hematoma.