Empirical Study On Brain Protection Of Rehmanniae Potion And Its Compatibility To Cerebral Ischemical Reperfusion Injury

Cerebral Infarction with various etiologies and complicated pathogenesis is the common and frequently encountered disease which seriously harms the health of human being,while Rehmanniae Potion is the effective prescription for treating the blood-lacking stroke.This topic studies the cerebral protection of Rehmanniae to ischemical reperfusion injury and Potion as well as its mechanism action.Besides,the topic also approaches the compatibility significance between the treatment of invigorating kidney and dissipating phlegm.Objective:To study the mechanism action and its compatibility significance on protection of Rehmanniae Potion to cerebral ischemical reperfusion injury.Method:Focal cerebral ischemia was induced by intraluminal middle cerebral artery (MCA) occlusion method using a nylon monofilament thread in rats. Rehmanniae Potion is separated into groups of invigorating kidney and dissipating phlegm.The neurologic impairment recuperation and cerebral infarction volume are observed with the score method and TTC staining method.HE staining and electron microscope were used to observe the changes of histomorphology.The expression of Bcl-2,NF-κBp65,TGF-β₁ and Nestin protein were measured by immunohistochemistry technique respectively.The expression of NestinmRNA,SCFmRNA,PSA-NCAMmRNA and TGF-β₁mRNA were measured by reverse transcription-polymerase chain reaction.Result:(1)Rehmanniae Potion has the obvious auxoaction on recuperation of neurologic impairment,and it can reduce cerebral infarction volume,lessen neurologic impairmen,repress apoptosis, promote Nestin, SCFmRNA,PSA-NCAMmRNA and TGF-β1 expression, which is of obvious difference with control group ( P<0.05).Groupâ… surpasses groupâ…¡(P<0.05)and the two groups have the synergistic effect.(2)Rehmanniae Potion can promote Bcl-2 expression and inhibit NF-KBp65 expression which is of obvious difference with the control group(P<0.05).Groupâ… surpasses groupâ…¡on the promotion of Bcl-2 expression(P<0.05),which has the correspondent action on NF-κBp65 repression with groupâ…¡,the two groups have the synergistic effect.Conclusion:(1)Rehmanniae Potion has the obvious auxoaction on neurologic impairment recuperation and reduces cerebral infarction volume which indicates its certain action of brain protection.(2)Rehmanniae Potion can lessen neurologic impairment, repress apoptosis,promote Bcl-2 expression,heighten TGF-β₁ expression and inhibit NF-κBp65 expression,which indicates that the action on repressing apoptosis is related to the regulation on Bcl-2,TGF-β₁and NF-κBp65 expression.(3)Rehmanniae Potion can promote Nestin expression,which indicates that it can promote the nerve stem cell proliferation and differentiation,while it can promote SCFmRNA,PSA-NCAMmRNA and TGF-β₁ expression, which indicates that the action of promoting the nerve stem cell proliferation and differentiation is related to the regulation on SCF,PSA-NCAM and TGF-β₁ expression.(4)Both invigorating kidney and dissipating phlegm treatment have the brain protection action and invigorating kidney treatment is superior, which indicates that the different treatments are not a simple addition but a synergistic action which hints the rationality of compatibility.