| Objective:The occurrence and development of the ischemical reperfusion injury of tissues is a process with synergistic action of multiple factor. A lot of reports have shown that it is possibly concerned with oxygen free radical, calcium overload and heterophil granulocyte.In the last several years,it is discovered by research that kinds of cell factors also participate with the physiopathologic process of hepatic ischemical reperfusion injury. These cell factors can cause the hepatic damage by the single way or by the dictyoid and synergistic way. In hepatic ischemical reperfusion injury,People have pay close attention to TNF-αwith the primary cell factor in Inflammatory reaction. It's widely recognized that TNF-αcan cause damages in the process of hepatic ischemical reperfusion injury,but it is discovered by research that the hepatocytes could be protected partly by making use of low dose TNF-αto make the preconditioning in before the process of hepatic ischemical reperfusion injury.Before the process of hepatic ischemical reperfusion 30 min,it could degrade the expression of TNF-αin hepatocytes and blood plasma after process of hepatic is chemic reperfusion and degrade the inspection level of ALT in blood serum and relieve hepatic damages obviously to give hepatocytess low dose TNF-α(1～5μg/kg weight).it is proved by Teoh that the hepatocytes could not be protected conspicuously to make the preconditioning to the mouse with gene knock-out, however, afer the mouse was intravenously injected low dose TNF-α(1ug/kg,imitating dose of TNF by the ischemic preconditioning),the protection of the hepatocytes was observed by the ischemic preconditioning in earlier period of the ischemic reperfusion(2h) and advanced stage(24h). Consequently,as a key factor,TNF regulate the protection of hepatocytess in ischemic preconditioning and promote the survivorship of hepatic cell in the ischemic reperfusion. So, we discuss initially the mechanism of the protection of hepatocytess in the ischemic reperfusion by immunohistochemistry and TUNEL,and supply the theory basis to research the mechanism of the protection of hepatocytess in the ischemic reperfusion.Methods:50 rats were made the hepatic ischemic reperfusion modelwe compared the preconditioning by THF with the ischemic preconditioning to analyze the protection of hepatocytess in the ischemic reperfusion .by observing morpholog changes of the hepatocytess ,detecting the level of ALT and AST by automatic biochemistry instrumental method, examining the proteinum expression level of Bcl-2 and NF-κB by immunohistochemistry and detecting the level of hepatic cell apoptosis by TUNEL.Results:the level of ALT and AST in the group with the preconditioning of TNF-αwas respectively 336.8±79.4 u/L and 392.7±109.3u/L, whereas the level of ALT and AST in the group with the ischemic reperfusion was 642.8±149.3u/L和730.6±103.0u/L, the difference in the two groups was significant (P<0.01). Cell index number was adopted to express the level of Bcl-2,and PI was respectively 60.99±6.30, 12.99±3.21 and 18.45±11.97 in the group with the preconditioning of TNF-α, control group and the group with the ischemic reperfusion.The former was higher than the two latter significantly, the difference was significant (P<0.05). Cell index number was adopted to express the level of NF-κB,and PI was respectively 62.80±6.30and 35.73±5.46 in the group with the preconditioning of TNF-αand the group with the ischemic reperfusion.The former was higher than the latter significantly, the difference was significant (P<0.05). Apoptotic index was respectively 4.94±2.04and 9.48±2.42 in the group with the preconditioning of TNF-αand the group with the ischemic reperfusion.The former was lower than the latter significantly, the difference was significant (P<0.05).there were no significant differences in the group with the preconditioning of TNF-αand the group with the ischemic reperfusion.Conclusions1 It was confirmed that the group with the preconditioning of TNF-αcould simulate partly the protection of hepatocytess in the ischemic preconditioning.The group with the preconditioning of TNF-αcould decrease the leakage of ALT and AST and elevate the expression of NF-κB and Bcl-2, thereby restrain apoptosis and relieve validly ischemical reperfusion injury.2 The expressions of Bcl-2 in all groups with the preconditioning were elevated,and between the expression of Bcl-2 and apoptotic index of hepatic cell showed significantly negative correlation.It was indicated that Bcl-2 had an important role in restraining apoptosis and relieving validly ischemical reperfusion injury.3 The expressions of NF-κB in all groups with the preconditioning were elevated,and between the expression of NF-κB and apoptotic index of hepatic cell showed significantly negative correlation.It was indicated that NF-κB had an important role in restraining apoptosis and relieving validly ischemical reperfusion injury.4 Between the expression of NF-κB and Bcl-2 showed no correlation.We considered that NF-κB maybe activated kinds of protective proteins and restrained apoptosis,but also activated the transcription of inflammatory factor in the ischemic reperfusion of hepatocytess.TNF-αhas diphasic action in the ischemic reperfusion of hepatocytess.So,from now on we should research deeply the mechanism of action of TNF-αand master the concrete directions,which has momentous significance to the explanation of the protection in the ischemic reperfusion and the development of the correlated medicine of TNF-αand the prevention and cure of ischemical reperfusion injury.
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