Neuroprotective Mechanisms Of Exercise In Mice Parkinsonism

Objective: To study the neuroprotective mechanisms of prior forced chronic treadmill running against the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) neuotoxic effect to mice. Contents: The making of mice MPTP Parkinson's disease model and the scoring of behavioral changes of the PD mouse; Measurement of the difference of mice brain mitochondria respiration capacity between groups; observation of morphological changes of SNpc; detection of the molecular biology changes of mice mice brain and liver. Methods: 32 male C57BL/6 mice were randomly assigned to either sedentary or exercise group. Mice in exercise group were forced to run on treadmill at moderate intensity (12m/min, 20minutes, daily) for 5 weeks. After that period of exercise, mice in either group were divided into two groups again randomly, and were administered moderate does of MPTP (2 30mg/kg×2,ip, 16 hr apart) or saline and at the end all animals were divided into the following groups: (1) saline; (2)exercise + saline; (3)MPTP; and (4) exercise + MPTP, 8 mice in each group. Three behavioral test: pole test traction test swim test were conducted in succession 1day prior to MPTP administration, and 2 5 8day after MPTP. Results Exercise and MPTP show significant interaction effects on all those tests,The Neuroprotective effect to mice brain can be mediated by following Mechanisms :1 improve the behavioral scores;2 raise brain mitochondria respiration capacity, more SNpc neurons were conserved; 3 stimulate more mRNA expression of various neurotrophins;4 downregulate the rate of Bax/Bcl2 mRNA expression; 5 increase more IGF-I to SNpc membrane. Conclusion :Increased expression of IGF-I and NGF mRNA in peripheral and central nervous system by Exercise can prevent SNpc neurons from apoptosis induced by MPTP via strengthening brain mitochondriarespiration capacity.