The Effect Of α-tocopherol In The Management Of SD Rat's Neurotrauma

Vitamine E is a common name of a series of molecules, includingα,β,γ,δtocopherols andα,β,γ,δtocotrienols. These molecules have the same chromanol ring, but they have different isoprenoid tails. Recent researches have discovered that Vitamine E is a natural antioxidant. Especially,α-tocopherol is an international generally accepted strongantioxidant,α-tocopherol can react with the oxidative free radicals(OFR), and formα- tocopherolquinone .α-tocopherol entrains the unconjugate electron from the OFR, and the OFR become the stable moleculars without the feature of oxidation. After neurotrauma, the damaged brain tissue produce great volume of OFR. The reasons of the production of OFR are: 1. XAN oxidase reacts with XAN 2. heavy metal ions like copper infiltrate into braintissue due to the damage of vascular system in brain. With the help of Vitamine C, these heavy metal ions can form great volume of OFR. 3. much PG and CA formation, and they lead to much OFR formation.4.after neurotrauma,anoxia and anemia exit in the damaged brain tissue. And the mitochondria of the neuro-tissue can't gain enough oxygen molecular. The electron can't be delivered effectively in the breathing chain, and the limited left oxygen molecules have to received more electrons. These oxygen molecules become the OFR then. OFR damage is a main reason for the secondary damage of the neurotrauma, and theα-tocopherol can eliminate the OFR damage. This research shows the contributive effect ofα-tocopherol on neurotrauma through the study of the neuron and the SD rats of neurotrauma. Part One. The Protective Effect of theα-tocopherol on NeuronAIM:compare the neurons underα-tocopherol protection and withoutα-tocopherol protection. Detect the the intracellular and extracellularOFR concentration,the stability of the cell membrane,the activity of the mitochondrial,the mRNA of the SAD and the micro morphous of the neurons to prove the protective function ofα-tocopherol on neurons.Methods: Culturing the neurons of the cortex of the SD embryo mice. Dividing the neurons into 2 groups:l.neurons underα-tocopherol protection (groupl);2.neurons withoutα-tocopherol protection(group2). Make the extracellualrα-tocopherol concentration of the group2 to 80mg/L. Produce OFR by Fenton reaction and damage the two groups of neurons. Detect the the intracellular and extracellular OFR concentration,the stability of the cell membrane,the activity of the mitochondrial,the mRNA of the SAD with ESR,confocal laser scanning microscope. Observe the micromorphous of the neurons with electronmicroscope.Results: comparing the two groups of neurons at different time points,we found that:l. the intracellular and extracellular OFR concentrations of groupl are lower than group2; 2. the stability of the cell membrane and the activity of the mitochondrial of groupl are better than group2;3.the volume of SDA mRNA of groupl is more than group2; 4.the damageof micro morphous of groupl is less than group2.Conclusion: This part of reseaech prove thatα-tocopherol can reducethe the extracellualr and intracellular OFR concentration of neurons,and reduce the damage of OFR to cell membrane,mitochondria,important enzymes and mRNA of the neurons。Part Two. The Protective Effect of theα-tocopherol on the SD rats's damaged brain tissue of moderate neurotrauma Aim:detect the OFR concentration,ICP,the concentration of active amino acid and the intracellular calcium cconcentration of the SD rats' brains to evaluate the contributive function of a-tocopherol to the mild neurotrauma of the SD rat.And evaluate the advantage and disadvantage of different ways of a -tocopherol administration.Methods:divide 256 250-300g SD male rats into 4 groups:group of microdialysis in brain tissue(groupl);group of intraventricular microdialysis(group2);group of intraperitoneal injection (group3) and group without a-tocopherol administration.Make the 4 groups rats mild neurotrauma with hydraulic pressure couping model.Continually observe the 4 groups of rats for 72h.Detect the OFR concentration,ICP,the concentration of active amino acid and the intracellular calcium concentration of the SD rats' brains at different time points.dye the pathologic slices of the rats's brains.Results:statistic the values of different indexes,we found that the OFR concentration,ICP,the concentration of active amino acid and the intracellular calcium concentration of the SD rats with a-tocopherol administration are less than the the SD rats without a-tocopherol administration.Comparing thegroupl,2 and 3,we found grouplshows the best therapeutic efficacy in the first 4hours after trauma,and group3 shows the the best therapeutic efficacy4hours after trauma.Conclusion: This part of research proves that a-tocophero can reduce the OFR production in the rat's brain,and reduce the ICP rising,the concentration of the active amino acid and the concentrations of intracellular calcium~ And the research also proves the contributive effect of a-tocopherol on neurotrauma by pathologic study.And it is also proved that intra brain tissue microdialysis and intraventricular microdialysis are feasible and effective ways of a-tocopherol administration.New ideas:1.Quantitatively detect of the intracellular and extracellular concentrations of OFR,and prove that a -tocopherol can reduce the the intracellular and extracellular concentrations of OFR of neurons.2.Through the continue photograph of micro laser scanning microscope,we find that the OFR damage the cell membrane at the junction of the axons and cell body of neurons firstly,and then damage the other position of the cell membrane.3.It is the first time to prove a-tocopherol can reduce the damage of OFR to the SDH mRNA.4.It is the first time to prove the intra brain tissue and intraventricular microdialysis of a -tocopherol are feasible and effective.5.We find time windiw of the intraperitoneal injection of a-tocopherol treatmemt.6.We find an unknown free radicals which is not mentioned in the literature.