| Gastric carcinoma is one common malignant tumors, lymph node metastasis is most cause of reoccur and bad prognosis. The mechanism of Gastric carcinoma occurring and lymph node metastasis is still unclear. Some oncogene, anti-oncogene and growth factors play important role in Gastric carcinoma growth and proliferation.PTEN is an anti-oncogene, PTEN protein suppress cell growth and induce apoptosis, inhibit tumor metastasis and invasion. IGF-1 and IGF-1R are important growth factor, promote cell growth and inhibit apoptosis. It have been reported that PTEN, IGF-1, IGF-1R involved in occurrence of Gastric carcinoma, but it is still unclear whether all of these three factors involved in occurrence and lymph node metastasis of gastric carcinoma, and their effect on prognosis is disputed.In the progress of tumor development, it shows heterology, and formed different subgroup, promote metastasis. The incidence rate and mortality rate of gastric carcinoma is very high, and one reason is metastasis. Research of gastric carcinoma heterology and PTEN, IGF-1, IGF-1R is rare.Recent work shows VEGFR-1+ bone marrow-derived hematopoietic progenitor cells involve in tumor metastasis, it locate to the pre-metastasis site and formed"niche"suitable for tumor cell cohere and growth. This research has been verified in lung cancer and melanoma, and concluded that all cell express CXCR4 can metastasis via this way. Gastric carcinoma also express CXCR4, it may extent by this way.The study investigate expression of PTEN,IGF-1,IGF-1R in gastric cancer tissue and regional lymph node by immunohistochemistry and reverse-transcription polymerse chain reaction. The aim was to explore the relationship between PTEN , IGF-1 , IGF-1R and gastric carcinoma clinicobiological characteristics, to assess roles of PTEN,IGF-1,IGF-1R in gastric carcinoma occurrence invasion and lymph node metastasis, to test the role of VEGFR-1 in tumor (gastric carcinoma) metastasis; enrich our understand of tumor, prepared for gastric carcinoma prevention and therapy.Partâ… Expression of PTEN,IGF-1,IGF-1R in gastric carcinomaObjective:Investigate expression of PTEN,IGF-1,IGF-1R in gastric carcinoma and the relationship with clinicobiological characteristics.Method:Investigate expression of PTEN,IGF-1,IGF-1R in gastric carcinoma tissue, gastric mucosa, analyze their relationship with clinicobiological characteristicsResults:1 Expression of PTEN,IGF-1,IGF-1R in gastric carcinoma and gastric mucosa. Expression of PTEN in gastric mucosa was strong, expression of IGF-1, IGF-1R was weak; expression of PTEN was weak in tumor, expression of IGF-1 and IGF-1R was strong. There was significant difference of PTEN IGF-1 IGF-1R positive expression between gastric carcinoma (40.5%,60.8%,63.5%) and gastric mucosa(75.0%,30.0%,35.0%) (P<0.01or P<0.05).2 Relationship between PTEN , IGF-1 , IGF-1R expression and clinicobiological characteristic in gastric carcinoma. 2.1 Relationship between PTEN expression and clinicobiological characteristic in gastric carcinoma. In gastric carcinoma PTEN expression was higher in without lymph node metastasis group(58.3 %) than lymph node metastasis group(23.7 %)(P<0.01,higher in stageâ… -â…¡group(60.9%)than stageâ…¢-â…£group(31.4%)(P<0.05.There was no significant difference in patients'age ,gender, tumor location, size, differentiation ,invasion depth(P>0.05). 2.2 Relationship between IGF-1 expression and clinicobiological characteristic in gastric carcinoma. In gastric carcinoma IGF-1 expression was higher in advanced group(65.6%) than early gastric carcinoma group(30.0%)(P<0.05),higher in poor differentiation group(84.6%)than moderate and well differentiation group(47.9%)(P<0.01);higher in lymph node metastasis group(78.9%) than without lymph node metastasis group(41.7%)(P<0.01)higher in stageâ… -â…¡group (39.1%) than stageâ…¢-â…£group (70.6%)(P<0.05).There was no significant difference with age ,gender, tumor location, size(P>0.05). 2.3 Relationship between IGF-1R expression and clinicobiological characteristic in gastric carcinoma. In gastric carcinoma,IGF-1R expression was higher in advanced group(67.2%) than early gastric carcinoma group(40.0%)(P<0.05),higher in poor differentiation group(80.8%)than moderate and well differentiation group(54.2%)(P<0.05);higher in lymph node metastasis group(84.2%) than without lymph node metastasis group(41.7%)(P<0.01), higher in stageâ… -â…¡group (39.1%) than stageâ…¢-â…£group(74.5%)(P<0.01). There was no significant difference with age , gender, tumor location, size(P>0.05).3 Relationship between PTEN,IGF-1,IGF-1R expression and prognosis. Prognosis of PTEN negative express Patients was poor compared with PTEN positive express Patients. Prognosis of IGF-1 IGF-1R positive expression Patients was poor compared with IGF-1 IGF-1R negative express Patients;Conclusion:1 Expression of PTEN is low in gastric carcinoma tissue, and expression of IGF-1 IGF-1R is high. There change maybe involve in gastric carcinoma occurrence.2 Expression of PTEN, IGF-1 and IGF-1R have relationship with gastric carcinoma clinicobiological characteristic, their expression can predict the malignancy of tumor.3 Expression of PTEN, IGF-1 IGF-1R can predict prognosisPartâ…¡Expression of PTEN,IGF-1,IGF-1R in gastric carcinomaObjective: To investigate expression of PTEN,IGF-1,IGF-1R mRNA in gastric carcinoma and there relationship with clinicobiological characteristics. Method:Investigate the expression of PTEN,IGF-1,IGF-1R mRNA in gastric carcinoma tissue, gastric mucosa ,analyze there relationship with RT-PCRResults:1 Expression of PTEN,IGF-1,IGF-1R mRNA in gastric carcinoma and gastric mucosa. There is significant difference of PTEN IGF-1 IGF-1R mRNA positive expression between gastric carcinoma (30.7%,59.1%,56.8%) and gastric mucosa 55.0%,30.0%,30.0%) (P<0.05).2 Relationship between PTEN,IGF-1,IGF-1R mRNA expression and clinicobiological characteristic in gastric carcinoma. 2.1 Relationship between PTEN mRNA expression and clinicobiological characteristic in gastric carcinoma. In gastric carcinoma PTEN mRNA expression was higher in early gastric carcinoma group(48.3%) than in progression group(22.0%)(P<0.05),higher in moderate and well differentiation group(45.2%)than poor differentiation group(22.8%)(P<0.05);higher in without lymph node metastasis group(41.5%) than lymph node metastasis group(21.2%)(P<0.05),higher in stageâ… -â…¡group(45.9%)than stageâ…¢-â…£group(19.6%)(P<0.01). 2.2 Relationship between IGF-1 mRNA expression and clinicobiological characteristic in gastric carcinoma. In gastric carcinoma, IGF-1 mRNA expression was higher in advanced group(67.8%) than early gastric carcinoma group(41.4%)(P<0.05),higher in poor differentiation group(70.2%)than moderate and well differentiation group(38.7%)(P<0.01);higher in lymph node metastasis group(70.2%) than without lymph node metastasis group(46.3%)(P<0.05),higher in stageâ…¢-â…£group (74.5%)than stageâ… -â…¡group (37.8%)(P<0.01). 2.3 Relationship between IGF-1R mRNA expression and clinicobiological characteristic in gastric carcinoma. In gastric carcinoma, IGF-1R mRNA expression was higher in progression group(66.1%) than early gastric carcinoma group(37.9%)(P<0.05),higher in poor differentiation group(68.4%)than moderate and well differentiation group(35.5%)(P<0.01);higher in lymph node metastasis group(68.1%) than without lymph node metastasis group(43.9%)(P<0.05), higher in stageâ…¢-â…£group(70.6%) than stageâ… -â…¡group(37.8%)(P<0.01).3 Correlationship between PTEN,IGF-1,IGF-1R expression PTEN mRNA expression have negative relation with IGF-1,IGF-1R mRNA expression, IGF-1 mRNA expression have positive relation with IGF-1R mRNA expression.Conclusion:1 Expression of PTEN mRNA is low in gastric carcinoma tissue, and expression of IGF-1 IGF-1R mRNA is high. There change maybe involve in gastric carcinoma onset.2 Expression of PTEN, IGF-1 IGF-1R mRNA have relationship with gastric carcinoma clinicobiological characteristic, their expression can predict the malignancy of tumor.3 Imbalance expression of PTEN, IGF-1 IGF-1R mRNA maybe involved in gastric carcinoma onset and progression.Partâ…¢Heterogeneity of expression of PTEN, IGF-1 IGF-1R in gastric carcinoma and regional lymph node.Objective: Investigate different expression of PTEN,IGF-1,IGF-1R in gastric carcinoma and regional lymph node.Method:Investigate the expression of PTEN,IGF-1,IGF-1R mRNA and protein in gastric carcinoma and regional lymph node by RT-PCR and immunohistochemistryResults:1 The expression of PTEN in gastric carcinoma and regional lymph node. Expression of PTEN in gastric carcinoma and regional lymph node with metastasis is strong, There is significant difference of PTEN positive expression between gastric carcinoma(33.3%),lymph node with metastasis (31.3%) and lymph node without metastasis ( 85.5%) (P<0.05).2 The expression of IGF-1 in gastric carcinoma and regional lymph node. Expression of IGF-1 in gastric carcinoma and regional lymph node with metastasis is strong, IGF-1 positive expression in gastric carcinoma and lymph node with metastasis is 77.8%, 81.3%.3 The expression of PTEN in gastric carcinoma and regional lymph node. Expression of IGF-1R in gastric carcinoma and regional lymph node with metastasis is strong, IGF-1 positive expression in gastric carcinoma and lymph node with metastasis is 83.3%,83.8%.4 Expression of PTEN, IGF-1, IGF-1R mRNA in gastric carcinoma and regional lymph node. Expression of PTEN, IGF-1, IGF-1R mRNA in gastric carcinoma and regional lymph node with metastasis have no significant difference, but expression of PTEN mRNA in lymph node with metastasis show degrade tendency and expression of IGF-1, IGF-1R mRNA show upgrade tendency .5 Relationship between PTEN,IGF-1,IGF-1R expression in lymph node and prognosis.Patients with IGF-1, IGF-1R mRNA positive express in lymph node have poor prognosis compare with patients with IGF-1, IGF-1R mRNA negative express in lymph node.Conclusion:1 There is Heterogeneity between gastric carcinoma and regional lymph node with metastasis.2 Expression of PTEN, IGF-1 IGF-1R in gastric carcinoma and regional lymph node with metastasis may have difference.3 Imbalanced expression of PTEN, IGF-1 IGF-1R maybe involved in gastric carcinoma regional lymph node metastasis.â…£The expression of VEGFR-1 in gastric carcinoma and regional lymph node.Objective: Investigate expression of VEGFR-1 in gastric carcinoma lymph node metastasis and distant metastasis.Method: investigate the expression of VEGFR-1 in gastric carcinoma, lymph node metastasis and distant metastasis by immunohistochemistry.Results: 1 Expression of VEGFR-1 in gastric carcinoma Expression of VEGFR-1 in gastric carcinoma is strong, positive express rate is 61.1%2 Expression of VEGFR-1 in lymph node metastasis Expression of VEGFR-1 in lymph node metastasis is strong, no significant expression of VEGFR-1 in lymph node without metastasis. VEGFR-1 positive express rate in lymph node metastasis is 56.7%3 Expression of VEGFR-1 in liver metastasis Expression of VEGFR-1 in lymph node metastasis is strong.Conclusion:1 Expression of VEGFR-1 in gastric carcinoma may promote cancer progression.2 Expression of VEGFR-1 in regional lymph node metastasis and liver metastasis increased.3 Expression of VEGFR-1 in stem cell around tumor metastasis indicted that VEGFR-1 may mediate tumor metastasis.
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