A Study On The Therapeutic And Preventive Effect And Mechanism Of Lycium Barbarum Polysaccharide On The Rat With Alcoholic Fatty Liver

With more and more people who are drinking heavily, alcoholic fatty liver (AFL) has already been a porular disease. AFL can be a serious damage to liver because it can lead to the fatty hepatitis and the liver fibrosis. By now, the pathogenesis of AFL is not well known, and there is still no effective medicine in treating and preventing the disease. So, to find an effective and safe Chinese traditional medicine has been our goal.CYP2E1 is an active producer of ROS, Which can be generated during its catalytic cycle even in the absence of substrate. This process, plus the induction of CYP2E1 by ethanol, has led to many studies on the role of CYP2E1-mediated oxidant stress in alcohol-induced hepatic injury. Lycium Barbarum Polysaccharide (LBP) is a very important antioxidant composition of LIXIA medlar, can help scavenging oxygen free radicals and inhibit lipid peroxidation and has widely been used as an active antioxidant, but its therapeutic and preventive mechanisms for liver disease are not well known. Aims of the study1. The establishment of experimental model of chronic alcoholic fatty life liver in rats.2. The mechanism of ethanol inducting AFL.3. The effect of LBP preventing and treating AFL.4. The mechanisms of LBP preventing and treating AFL.Methods1. The AFL rat models were established by ethanol intragastric infusion in ten weeks.2. Selected samples of the left liver from each rat were rapidly removed at random and fixed in 10% neutral buffered formalin. The sections were stained with hematoxylin and eosin(HE) and SuDanⅢ, and the histopathological analysis were made.3. The rat Serum ALT, AST,γ-GT levels were evaluated using an assay kit.4. Selected samples of right liver from each rat were rapidly stored under 4℃for use. The protein content was estimated by the dye-KaoMaShi, GSH-PX,SOD,MDA,GSH,H2O were evaluated according to the manual of assay kit.5. The hepatocyte CYP2E1 gene and protein expression were detected using Reverse-Transcriptase Polymerase Chain Reaction(RT-PCR) , Immunstaining and Westen Blot.6. The hepatocyte CYP3A activity and gene expression were detected using Nash method and Reverse-Transcriptase Polymerase Chain Reaction(RT-PCR) respectively.Results and conclusions1. Wister rats were treated with 40% (V/V) ethanol 8g/Kg intragastric infusion twice a day to the end of the fifth week , then, from the beginning of the sixth week, treated with 50% ethanol(V/V)9g/Kg.d intragastric infusion twice a day to the end of the tenth week.Thus, the experimental animal model of chromic alcoholic fatty liver was successfully established.2. Ethanol-induced lipid peroxidation is can be a major role in mechanisms by which ethanol causes liver injury.Ethanol could induce oxidative stress, ruduce the activity of GSH-PX, SOD and the content of GSH and could also increase the content of MDA and H2O2 through inducing the hepatocyte CYP2E1 gene and protein expression and the CYP3A activity and gene expression.3. LBP could reduce the activity of serum ALT, AST andγ-GT, and the content of MDA, H2O2, increase the activity of GSH-PX, SOD and the content of GSH.LBP can effectively prevent and treat AFL by inhibit the expressions of CYP2E1 gene and protin and the expressions of CYP3A gene and its activity.