| Objective:To observe the effects of the Biejiajian pill on rats of diabetes with atherosclerosis, blood lipid, blood sugar, vascular endothelial factor, inflam-matory factor, anti-inflammatory factors, etc. and to inve-stigate its possible mechanism of action.Methods: The modeling male SD rats were divided randomly into: model group, Tongxinluo control group, low dose of Biejiajian pill group, and high dose of Biejiajian pill group. The same week-old rats are blank control group. After 8 weeks, drawing blood and taking surrounding fat tissue of testis, pancreas, and thoracic aorta. Examination:①Blood lipid: TC, TG;②Inflammatory factor: CRP, TNF-α;③Diastolic-systolic Factors of Vascular Endothelium: ET, NO;④Anti-inflammatory factors: serum adiponectin. Measuring the level of adiponectin mRNA in testis white fat by RT-PCR;⑤pathological section: to observe pancreatic gland and atherosclerotic lesion of aortic intima with microscope and to measure the level of MMP-9 by immunohistochemistry.Results:①The influence on Blood sugar, blood lipid: blood sugar in model rat is higher in all terms; compared with model group , high dose of Biejianjian pill and low dose of Biejianjian pill can decreased the level of TG(P< 0.01), and have no difference among each therapic group(P>0.05); the level of TC have no change among each therapic group compared with model group(P>0.05).②The influence on inflammatory factor: compared with blank control group, the level of CRP, TNF-αincreased in model rats(P<0.05), decreased in each group of Biejiajian pill group compared with model group and the difference was significant(P<0.05), the level have no difference among each therapic group(P>0.05). Immunohistochemistry indicate that the level of MMP-9 in rats of the model group were higher than blank control group and the difference was significant(P< 0.01). compared with model group, The level of MMP-9 in rats of low and high dose of Biejiajian pill group can be reduced, the difference of high dose was significant(P < 0.01). and the difference were insignificant among each therapic group(P>0.05).③The influence on diastolic-systolic Factors of vascular endothelium: compared with blank control group, the level of ET increased in model rats(P<0.05), decreased in each group of Biejiajian pill group compared with model group and the difference was significant(P<0.05 or P<0.01), the level have no difference among each therapic group(P>0.05). The level of NO was lower in model group compared with blank control group(P<0.01), increased in high dose and low dose Biejiajian pill group and the difference were markedly(P<0.05), the level have no difference among each therapic group, and the difference was insignificant(P>0.05).④The influence on anti-inflammatory factors: compared with blank control group, the level of APN and APN mRNA decreased in rats of model group(P<0.01), increased in each group of Biejiajian pill group compared with model group and the difference was significant(P<0.01 or P<0.05), the level have no difference among each therapic group(P>0.05).⑤The influence on pathological section of pancreatic gland and atherosclerotic lesion of aortic intima: Compared with blank control group, we can see less pancreatic islet, smaller pancreatic islet and less cell in pancreatic islet in the tissue of pancreatic gland among model group and therapic group. Under microscope, aortic intima have xanthoma cell aggregation, aortic intima thickening, lipid deposition, proliferative smooth muscle cell, interstitial collagen hyperplasia, etc in model group and therapic group .But it is more markedly in model group.Conclusion: Biejiajian pill can reduce the levels of TG, CRP, TNF-α, ET, MMP-9, and increase the level of NO, serum adiponectin, adiponect mRNA, and so improve endothelial function and anti-inflammatory action. Biejiajian pill has effect on experimental rats of diabetes with atherosclerosis in protecting vessel, whose mechanism may be relation to protection of endothlial function and anti-inflammatory mechanism.
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