Effect Of Reinforcing Earth To Strengthen Metal In Rat Model Of Allergic Rhinitis With Speen-deficency

OBJECTIVE:Allergic rhinitis is one of the most prevalent diseases. It has significant effects on quality of life. there are no known interventions that can cure allergic inflammation, and the management is frequently symptomatic. Traditional Chinese medicine coumpound is concluded from Pro. Xiong Dajing's long-clinic experience and has reliable treating effect on allergic rhinitis. We observe the effect of reinforcing earth to strengthen metal on Allergic rhinitis (AR) in Spleen Qi deficiency type and the mechanism of reinforcing earth to strengthen metal in treating this disease from gene level. To establish the clinic treatment principle tonify Spleen Qi and reinforce Surface-of AR through the Lab. Research from pathogenic, physiologic changes.METHODS:Establishing rat's AR model or AR model in Spleen Qi deficiency type,7 groups are involved which are the normal group, the Spleen Qi deficiency AR group, the Spleen Qi deficiency AR Chinese medicine group, the Spleen Qi deficiency AR Western medicine group, the AR group; the AR Chinese medicine group and the AR Western medicine group. Establish the rat's Spleen Qi deficiency modal with improper diet and over strain according to Li Dexin's report, AR modal according to An Yunfang's. After the model was established, Traditional Chinese medicine coumpound or loratadine is given to the rat by enema starting from 23rd days to 29th days. During, the treatment phase, the rats were stimulating by 0.5% OVA 1 hour after the medicine administrate, the author observed the symptoms especially nasal symptoms. After treatment, author apply the Light Microscope to observe the pathogenic changes happened in the model rats, and choice 6 rats to apply RT PCR to observe mRNA, such as IL-4 mRNA, IL-5 mRNA, IL-13 mRNA,Eotaxin mRNA and Stat-6 mRNA. Additionally, the serumal content of IgE was determined by ELISA. RESULTS:Reinforcing earth to strengthen metal could significant functions of control the symptoms, low down the higher IgE in blood,reduce the pathologic changes of nasal mucosa. Deduce the expression of IL-4 mRNA; IL-5 mRNA; IL-3 mRNA; Eotaxin mRNA and Stat-6 mRNA. Compare to rat's AR modal, the AR rats in Spleen Qi deficiency type more severe manifestations and pathogenic changes, the expressions of IL-5 mRNA; IL-3 mRNA; Eotaxin mRNA have increased.CONCLUSION:Symptom of AR, such as sneezing, rhinorrhea and grabbing nose frequently were controlled by Chinese medicine of reinforcing earth to strengthen metal. Reinforcing earth to strengthen metal can decrease the.number of eosinophil in nasal secretion of nose and the level of IgE and reduce level of gene expression of IL-4 mRNA, IL-5 mRNA, IL-13 mRNA, Eotaxin mRNA and Stat-6 mRNA of AR guinea pigs. This is probably the main mechanism of anti-AR. The syndrome of traditional Chinese medicine of deficiency of Spleen Qi would aggravate the pathological state of AR.