The Effect Of UNDJ On Neural Regeneration Signaling Protein GAP-43,Rho During Cerebral Infarction Convalescence

Objectives:Study on the effect of UNDJ on neural regeneration signaling molecule GAP-43,Rho during cerebral infarction convalescence to provide the possible anti-ischemic mechanism.Methods:1. Through the collection of ancient and modern literature, review the process of prevention and treatment on ICVD both in Western and Chinese medicine. Study the modern pharmacological action of classical decoction Buyang Huanwu. Have a preliminary grasp of some signaling molecules on neural regeneration.2. Rat focal cerebral ischemia was induced by MCAO, After Rats model of focal cerebral ischemia induced, All adult male SD rats were randomly divided into 7 groups including naive, shame-operated, ischemic, ischemic with BYHW, ischemic with NDJ, ischemic with UNDJ and ischemic with 1/3UNDJ groups. Each group have 20 rats. ALL the rats were given different treatment and were killed in 1,3,7or14day after operation. To observe the infarct size by TTC and evaluation the nerve function, the expressions of GAP-43,Rho were measured by immunohistochemical staining both with RT-PCR.Results:1. Literature Study:ICVD is a common complaint among the elderly.1.1 Pattern of qi deficiency and blood stasis is often seen in traditional Chinese medical science, qi deficiency is etiological factor while blood stasis is symptomatic factor. We replenishing qi and activating blood in order to dispelling stasis to promote regeneration.1.2 Cerebral thrombosis is often seen in western medicine, Obstruction of a blood vessel and hemorheology by an embolus usually can be seen. Modern medicine shows that the main therapeutic goal during cerebral infarction convalescence is to help the centrum neural regeneration.But now all the treatment effects are have deficient and below the mark.1.3 BYHW have many modern pharmacological action to improve the neurological deficit.1.4 GAP-43 is a internationally recognized neural regeneration signaling protein,and small G-protein Rho is one of the latest rejection capability neural regeneration signaling molecule. 2. Experimental Study:2.1 After ischemia, the pale infarction in hemorrhage brain can be seem greater than the brain offside by TTC detection。2.2 All the pathological sections with NDJ treatment groups show a improved tendency.2.3 All the NDJ treatment groups can significant improve neurological deficits at 3,7,14days (P< 0.05), the 1/3UNDJ group,BYHW group and decoction NDJ group have no obvious differences at each time point (P>0.05)2.4 Immunohistochemical staining showed that the GAP-43 expressions level in all treatment groups is higher than the ischemic groups at each time point, there was a significant difference(P<0.05).2.5 RT-PCR showed that the GAP-43 mRNA level in all treatment groups is higher than the ischemic groups after 3d time point, there was a significant difference(P<0.05). the 1/3UNDJ group,BYHW group and decoction NDJ group have no obvious differences at each time point (P>0.05)2.6 RT-PCR showed that the Rho mRNA level in all treatment groups is lower than the ischemic groups after 3d time point, there was a significant difference(P<0.05). the 1/3UNDJ group,BYHW group and decoction NDJ group have no obvious differences at each time point (P>0.05) Conclusions:1. Decoction NDJ can improve the neurological deficits.2. NDJ can promote GAP43 expression while resistant Rho expression after cerebral ischemia of MCAO rats,which maybe one of anti-ischemic mechanism of the drug.3.1/3 UNDJ is affected with a considerable amount of the role of traditional pieces.