| Objective:Senility is the bad change on all levels of multicellular organism and tissue along with the time, and represents the letdown of the function, adaptability and resistibility. On humankind, it represents a series of recessive change on aspects of physiological and morphological. Stress, which has been put forward in 1930s by Han Salye, is a all-around responsive state under stimulation of huge change on inside and outside circumstance. It includes the responses of energy, nerve, incretion, immunity and so on.Since the Twentieth Century, accompanying with the development of socioeconomy and technology, expectation of mankind's life is protracting, the world population frame become elder, and a old era is coming. At the appointed time, huge fee-for-service of elder will be a heavy burden on every country. And the only way for this problem is advance the health of the elder. At the same time, high efficiency and fast rhythm of modern society aggravates the psychological burthen of mankind, makes us be in the universal stress. The factors such as mentality, social and circumstance affect the human's health badly, accelerate the process of senility. Discussing the pathogenic and physiological mechanism of emotion and stress leading senility under the model of "biology—psychology—community", fall the damage of stress to the elder and prolonging life are help to open out the nature of senility in-depth, to develop the theory of Herbalist doctor and to exploit new fields of prolonging-life.In china, the acupuncture and moxibustion has watched a long history in senility-resistance, especially the catgut embedment in acupoint is save-time, softness and long-haul. It is of unexampled advantage on falling the stress damage, preventing and curing geriatrics and prolonging life. So this research will offer a new mind and means for those.The research has created an aging rat's mode which results from multiple factors. Call it multiple aging rats'mode. In addition, this research reveals different aging between multiple rat mode and glactosum mode degrees related indexMethods:1 Research based on literaturePresenting Chinese traditional anti-aging theory in various aspects, which probes deeply into relativity between traditional anti-aging transaction and modern doctrinal about anti-aging, this is based on literatures which are published recent 20 years. By summarizing traditional anti-aging methods and transaction of acupuncture for anti-aging, this paper will give specific guidance, meanwhile, accomplish analyses, together with discussion for modern literature review。2 Research based on experimentCatgut Embedment therapy stimulate certain part to distinguish pallium and hippocampus in 2 kind of condition, that one is normal while the other is under chronic stress in neuroendocrinology aspect of glactosum ethology, thymus exponent, HPA axis。This research is providing it scientific evidence, which is discussing chronic stress that facilitates the anti-aging process and Catgut Embedment commendation。(1) Grouping:Animal used as test and grouping:Using random number list, divides all animals in to 4 groups:Blank group (NS intraperitoneal injection), model group 1(glactosum intraperitoneal injection), model group 2 model (glactosum intraperitoneal injection, braking acousto-optic laser), group 3(glactosum intraperitoneal injection, braking acousto-optic laser, Catgut Embedment) 12 rats/group.(2)Modeling methods: Blank controller (groupl):equivalent NS intraperitoneal injection daily, continually for 6 weeksGroup 2:equivalent 5%D-glactosum (120mg/kg/d) intraperitoneal injection daily, continually 6 weeksGroup 3:equivalent 5%D-glactosum (120mg/kg/d) intraperitoneal injection daily, for six weeks; From the third week braking stimulation for 4 weeks; from the third week acoustic noise stimulation 30min/day, for 4weeks; From the third week photostimulation reverse day and nightfor 4weeks.(3)Embedment method:According to the traditional theory of spleen and kidney under TCM and sphycological academic thought, we select "shenshu" "baihui" as prescription 1, " zusanli and mingmen "as prescription 2. one time a week,5 times in total, embedment method therapy (4)Observe general behavior such as weight, hair, spirit, dining and movement.(5) By observing maze experiment, we can tell differences in cognitive ability among different groups.(6)To test the difference of rats'thymus splenic index, observe the change in immune ability.(7) Measure SOD activity degree and content of MDA in rat blood. Observe the changing of radical in rats.(8) Measure 3 kinds of hormones serum cortisol, serum ACTH, hypothalamus CRH. observe the changing of HPA s-function in all groups.(9) Find out the difference of receptor expressions'genes of Hippocampus glucocorticoid by using the PCR technology(10)After analyzing related index above we can find out potential relation to probe into the clinical result of catgut embedment in acupoint and its transaction.3,Result and Conclusion(1)There is abundant experience on anti-ageing, prevented illness and prolonging life in TCM. To use the acupuncture and moxibustion to delay ageing process is very special and strong.It is of many advantages and takes the lead in such way:cheap, easy and non-poison etc.(2)On general behavior, the senile model and stress model all appeared with listless spirit, lumberly movement, weight increase getting slow, their color turned reluster and appetite reduce. After stressing, the senile become more obviously. Catgut embedment in acupoint could reduce the ageing process on the experimental senile rats.(3) On spontaneous behavior maze experiment, group 2 is slower to find the way than group 1. After stressing, that becomes obvious. But after Catgut embedment in half of rats in group 2,result come that rat with catgut therapy use less time to find way comparing to the rest of the rats in group 2. That means Catgut embedment in acupoint could improve the ability of spontaneous behavior to resist senile.(4)On thymus and splenic index, index of group 2 is lower than that of the blank group. After stressing, it becomes obviously. It can be explained that the chronic stress can accelerate the senile. Catgut embedment in acupoint could hoist the thymus index to resist senile.(5)On the change of HPA axis function, the senile group's blood plasma cortex is higher than that of the blank group obviously whereas its blood plasma ACTH and thalamencephalon CRH are close to those of young rats. After stressing, that become obviously. It shows that senile and chronic stress can make the HPA axis function become sthenic and accelerate senile. Catgut embedment in acupoint could decrease the level of blood plasma cortex, blood plasma ACTH and thalamencephalon CRH as to resist senile.(6)The observation on pallium by optical microscope shows that the senile group appears with some karyon solid crinkle. After stressing, that become obviously, and cells decrease obviously. Catgut embedment in acupoint could lighten the degree of solid crinkle.(7)On pyramidal cells counting, the pyramidal cells amounts of model group and model + stress group are lower than that of the blank group obviously, and the model + stress is the lowest. But catgut embedment in acupoint could make the higher amount.(8)The observation on hippocampi ultrastructure shows that the senile rat's nerve cell dwindled, the cytochylema organelle reduced. After stressing, the cells become iiregular, the organelle reduced obviously, even vanished. Catgut embedment in acupoint could improve it(9)The senile group's function of nerve, incretion, immunity and resist stress become disabled, and the long time chronic stress aggravated it. Catgut embedment in acupoint could adjust those functions to resist senility.(10)To observe the aging process on behavior, immunity, nerve incretion foul-up and brain senescence, use the catgut embedment in acupoint theory to improve those functions to resist senile. And provide science gist for clinical using of catgut embedment in acupoint to resist senility.
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