The Impact Of Quercetin, Liverwort Flavone And Dads On The Proliferation And Apoptosis Of Hepatic Cancer Cell Hepg2 As Well As Some Related Research On Their Acting Mechanism

It has been known that quercetin and DADS can restrain the growth of many kinds of cancer cells; liverwort has been reported to contain anti-cancer and cancer-reversible ingredients, but their research results have had no literary reports, as the active ingredients in quercetin, DADS and liverwort have low toxic and side-effect, but with extensive application prospects, therefore the research about them has become the focus of the medical field. However, there has been few deep and systematic research about their acting mechanism, not to mention the report about the research of the compatibility value of the active ingredients in the above mentioned traditional Chinese medicine.This research take HepG2 cells as its subjects, with such experiments undertaken, such as, cell experiment in vitro, model experiment of transplanted tumors inside nuid mice. By applying such technologies as cell biology, molecular biology and morphology combined together, the research is intended to explore the impact or effects of medicinal ingredients from quercetin, DADS and liverwort flavone as well as their mixtures on HepG2 cells. Moreover, their possible inducing action and mechanism on the apoptosis of HepG2 cells have also been studied in this research.The research results show that quercetin, DADS and liverwort flavone as well as their mixtures all can induce the apoptosis of HepG2 cells, and is also closely related to the increased phosphorylation expression of p38 MAPK. In the experiment of human liver cancer cells-HepG2 cells transplanted into nuid mice, it has been found that DADS and liverwort flavone both could apparently restrain the proliferation of transplanted tumors in nuid mice, the higher the density of DADS and liverwort flavone, the stronger restraint on the proliferation of transplanted tumors in nuid mice. However, when DADS and liverwort flavone are separately implanted into nuid mice who have been transplanted cancer tissues, it has been shown that the count of cell apoptosis has also obviously increased. In immunohistochemical mothod and Western-blot experiments also exhibited that many significant factors from the cell conducting paths for information access have jointly contributed to the apoptosis process of human liver cancer cells-HepG2 cells because of the induction of DADS and liverwort flavone. In addition, as is found when the research on cellular level was done, there is increased phosphorylation expression of p38 MAPK when DADS and liverwort flavone are separately implanted into naked rats who have been transplanted cancer tissues, and the expression of BCL-2, Caspase-3 and PCNA has also been affected. Through the experiment of transplanting human liver cancer cells-HepG2 into the naked rats, it has been concluded that DADS has played a role in the apoptosis process of HepG2 cells at individual cellular level. As for the research on the mechanism of apoptosis of HepG2 cells, it has been discovered that phosphorylation of p38MAPK protein is one of the important means of inducing the apoptosis of HepG2 cells. Meanwhile, with Capase-3 activated and the expression of BCL-2 and PCN protein decreased, the the apoptosis process of HepG2 cells has been sped up, so that the aim of restraining the growth of tumors has been achieved. Thus this research will surely provide vital evidence of experiment for the clinical application of the medicinal ingredients in the above mentioned traditional Chinese medicine.