Effects Of Tang Zhi Qing On Diabetic Pancreas βcell Function In Rats And Possible Mechanisms

Objective:To observe Tang Zhi Qing in type 2 diabetic rat model of blood biochemical parameters, pancreatic histopathology and ultrastructure, and to explore its mechanism.Methods:In fed with high fat and sugar diet based on low-dose streptozotocin rat islet (3-cell damage, resulting in type 2 diabetic rat model.After the successful model of type 2 diabetic rats were divided into model group (no drug intervention), Tang Zhi Qing high dose, low dose treatment group, pioglitazone intervention group and control group. After 4 weeks the rats were observed the following indicators:①insulin resistance index, glucose utilization (GIR);②inflammatory cytokines, such as serum high sensitivity C-reactive protein, tumor necrosis factor and serum adiponectin;③oxidative stress parameters (serum MDA, SOD and GSH-PX, etc.);④IRS-1 mRNA expression;⑤isletβcell apoptosis;⑥islet histopathological and ultrastructural examination.Results:Diabetic group compared with the normal group, Body weight in rats, fasting glucose, total cholesterol, triglycerides, LDL-C, HDL-C, fasting insulin levels were significantly different (p<0.05), suggest successful model. After 4 weeks treatment Tang Zhi Qing each dose group, pioglitazone group compared with the diabetic model group:①insulin resistance index decreased and glucose utilization (GIR) were increased; There was a significant difference (p<0.05).②high-sensitivity C-reactive protein and TNFa were significantly decreased (p <0.05), Serum adiponectin were significantly increased.③Diabetic control group was significantly higher in pancreatic tissue MDA, and SOD, insulin levels were significantly lower than the control group (P<0.05).Different doses of Tang Zhi Qing treated group after treatment, serum SOD, MDA, insulin values before treatment were significantly different (P<0.05), Compared with the diabetic control group were also significantly different (P<0.05).④normal rat islet IRS-1 mRNA expression levels, diabetes mellitus model group was significantly lower than normal expression (P<0.05), The Tang Zhi Qing low dose group, Tang Zhi Qing high dose islet IRS-1 mRNA ratio than the disease model of diabetes was significantly higher insulin (P<0.05), Equal to or higher than the pioglitazone group.⑤islet cell apoptosis observed:diabetic group the largest number of positive staining cells in the normal control group, the smallest number of positive stained cells;Tang Zhi Qing low dose group the number of positive staining cells are more insulin and diabetes disease model group was not statistically significant (P> 0.05), Pioglitazone group and Tang Zhi Qing high dose group the number of positive staining cells was significantly reduced compared with diabetic group, was statistically significant (P<0.05).⑥Light microscope HE staining in the normal control group showed islets rich in complete form;diabetic rat pancreas, exocrine decrease in the number of islet cells (+-++);Pioglitazone group:reduction in the number of islet cells (+); The low and high doses of Tang Zhi Qing administration of treatment, the rats were improved pancreatic morphology;Tang Zhi Qing morphology close to the high dose Pioglitazone group. Electron microscopy and light microscopy findings were similar.Conclusions:①Tang Zhi Qing could reduce fasting glucose. the protection of pancreatic P cells Mechanism may be by increasing the islet glucose transporter 4 gene expression improved glucose and lipid metabolism, reduce levels of inflammatory cytokines and increased serum adiponectin levels, reduce low-grade inflammation and inhibition of oxidative stress, thereby reducing glucose and lipid toxicity, increased insulin Sensitivity and improve insulin resistance andβcell apoptosis and reduction of coagulation and fibrinolysis system reduces PAI-1 levels, improve blood stasis condition and other aspects to achieve.