The Effect Of Profit Kidney Healthy Bone Pellet From Body Bone In Repairing Bone Damage And The Expression Of IGF-1

Objective:To investigate The effect of traditional Chinese medicine from body bone in repairing bone damage and the expression of IGF-1 (insulin-like growth factor-1, IGF-1); Methods:Experiment domestic rabbit 90, female and male do not limit, body weight (2.0±0.5) kg. After the surgery, divides into the experimental group, the effective control group and control group each 30 stochastically. Separately after the surgery, whenh 2,4,6,8 week each group executes 6 domestic rabbits stochastically, takes the domestic rabbit radius bone damage place specimen examination. Experimental group, effective control group and control group after ketamine 30mg/kg intermuscular injection anaesthesia, according to the aseptic technique technology line left side in the radius on the croppings bone, creates the 15mm bone and the periosteum damage, the medulla cavity with the bone sacrificial seal, in addition takes two-sided makes from the body qia bone 1×1×2mm the granular bone, enters the above granular bone in the bone damage spot planting. Wounds sutures strictly, does not do inside and outside fixed, divides the cage to feed. After the technique, experimental group the traditional Chinese medicine mixture which with the velvet, obscene Yang Huo, the Eucommia, the leech, the davallia, the sonchus asper, the native copper, the woodlouse makes "the profit kidney healthy bone pellet" the feed feeds, the effective control group meets blade's feed with the mix traumatology department to feed, the control group with does not feed including the traditional Chinese medicine ordinary feed, free activity, after the technique, the intramuscular injection celebrates the magnamycin 3 days, the prevention infection. After domestic rabbit technique, separately in 1,2,4,6,8 week fashionable radius X-line inspection, and takes the domestic rabbit radius bone damage place organization to take the specimen, concrete observation and analysis "profit kidney healthy bone pellet" from body bone repair bone damage in intervention effect and insulin type growth factor 1 (IGF-1) gene expression. Results:X-Ray observation:When the 1st week, the experimental group^ the effective of the control group and the control group had no significant changes in restoration area, you can see the bone defect with bone graft granular shadow, clear boundaries, no new bone formation of Callus; When the 2nd week, the experimental group and control group were effective beginning with a small amount of Callus formation, bone clearer boundaries, the control group of bone Callus, but also slightly less than the experimental group and the effective control; When the 4th week, the experimental group and effective group both had Callus formation which was significantly increased, the experimental group compared to the effective control flaky little Callus, while the control group only increased the amount of bone Callus bone Callus no sheet; When the 6th week, the experimental bone defect fuzzy group, bone defects can be seen woven bone hypertrophy, more effective control group had a small amount of cartilage and woven bone island, while the control group, the bone defect can only see the scattered islands of cartilage; When the 8th week, the effective control group and the formation of large bone defects Callus, into a higher density of diffuse shadow, and the experimental group, bone marrow cavity formation but some of the control group 8 weeks through yet another sign of the bone marrow cavity; and the group Although the bone defect area of the same Callus formation, but the experimental group than in terms of, the amount of Callus formation and effective control for at least the control group was relatively small.Histological observation:Experimental group and effective control group and control group when the 1st week transplants in the bone to have the part of bone cell necrosis, the cell nucleus concentration, even to vanish, transplants in the bone to have the ciliary cell proliferation and to be accompanied by the inflammatory cell production; when 2-4 weeks the bone cerebral cortex long enters transplants in the bone and has the few cartilage cell formation, simultaneously the experimental group is accompanied by the massive blood vessels to grow, transplants in the bone the cell to absorb and has the nucleus to concentrate the disruption phenomenon obviously, the effective control group is accompanied by many blood vessels to grow, transplants in the bone the cell absorption and also presents the nucleus concentration disruption phenomenon; when 6-8 weeks transplants the bone area blood vessel quantity to increase, the experimental group has the obvious netted new osteogenesis, and is accompanied by the medullary cavity of bones the formation, the effective control group obvious lattice new osteogenesis, and is accompanied by the few medullary cavities of bones the formation. When control group 1 week the transplant area also has the massive inflammatory responses to change not obviously with the experimental group; But 2nd-4th week, later and is accompanied by the few cartilage cell and the vascularization; When the 6th-8th week, group the Callus quantity increases and is accompanied by blood vessel's increase, but not yet forms the medullary cavity of bones. The electron microscope observation:When the 1st week, the experimental group, the effective control group and the control group have part of bone young Liang to destroy necrosis shape, and is accompanied by the many inflammatory cell's productions; When the 2nd week, the experimental group cell assumes spindle-shaped, the surroundings have the few textile fiber ingredient, the bone cell nucleus concentration, the chromatin condensation keep to the side, the effective control group also presents the spindle-shaped cell; The control group has not seen the spindle-shaped cell production, around the bone cell the obvious collagenous fibre, the paralinin keeps to the side compactly; When the 4th week, experimental group blood capillary quantity increases, the bone cell bone dimple and bone young Liang forms, effective control group blood capillary quantity also increases, but the bone cell bone dimple and bone young Liang forms are relatively few; But control group blood capillary quantity are relatively few and around the partner has the bone matrix formation; When the 6th-8th week, the experimental group osteoblast formation, the experimental group ossein and the bone cell nucleolus are quite obvious, obviously in the butcher nature endoplasmic reticulum quantity increases obviously, the effective control group obvious ossein and the bone cell nucleolus appear, obviously in the butcher nature endoplasmic reticulum quantity increases relatively, but control group osteoblast quantity are few, ossein surface shaping unit bone cell bone young Liang and bone canals. Immunity chemical staining observation:When the 1st week, the control group, the effective control group and the experimental group IGF-1 dyeing sees the bone matrix, the bone cell and the cytoblastema assumes the diffused to dye pale, but each group distinguishes not obviously, assumes the weak masculine expression; When the 2nd week, the experimental group bone cell and the bone matrix dyeing deepens, the IGF-1 dyeing assumes the masculine gender to express, the effective control group bone cell and the bone matrix dye also the visible part to deepen, assume the weak masculine gender to express, but the control group still presented proliferatingly dyes the expression pale; When the 4th week, the experimental group IGF-1 dyeing assumes the strong masculine gender to express, follows the medullary cavity of bones the formation, the bone matrix, the bone cell coloration is deep, the cartilage cell and the blood capillary also obviously thickly dye; The effective control group IGF-1 dyeing assumes the masculine gender to express, follows the medullary cavity of bones the formation, the bone matrix, the bone cell coloration; But the control group also dyes deepens but the density slightly pale in the effective control group, the experimental group, assumes the weak masculine expression; When the 6th-8th week, before each group of bone cells, the bone matrix and bone young Liang's IGF-1 dyes, assumes the corresponding degree to weaken the expression, the control group assumes the weak masculine gender to express, the effective control group assumes the masculine gender to express, the experimental group still assumed the strong masculine expression. Situhybridization observation:When the 1 st week, the control group, the effective control group with the experimental group not obvious difference, the IGF-1 probe dye the matrix coloration to assume the weak masculine expression; When the 2nd week, the experimental group IGF-1 probe dyes the matrix coloration to assume the masculine expression. The effective control group IGF-1 probe dyes the matrix coloration to assume the weak masculine gender to express, visible part bone matrix and bone cell coloration. But the control group IGF-1 probe dyes the matrix coloration effective control group slightly little to assume the weak masculine expression. When the 4th week,the experimental group IGF-1 probe dyes the matrix coloration to assume the strong masculine expression. The effective control group IGF-1 probe dyes sees bone young Liang to form, and bone young Liang and the bone cell coloration assume the masculine expression. But the control group IGF-1 probe dyes the matrix coloration to assume the weak masculine expression. When the 6th-8th week, each group may see that massive bone young Liang forms, before the bone cell number also increases, before but regardless of is the bone matrix, the bone cell, bone young Liang the IGF-1 probe dyes the matrix coloration expression, to start to weaken. The effective control group IGF-1 probe dyes the matrix coloration to assume the weak masculine expression. But the control group IGF-1 probe dyes the matrix coloration effective control group to express weakens. The experimental group IGF-1 probe dyes the matrix coloration to assume the masculine expression. RT-PCR result:IGF-1 the gene mRNA relative expression quantity assumes the trend of escalation in the 1-4th week, the 6-8th week assumes the declining trend. The experimental group and effective control group IGF-1 the gene mRNA relative expression quantity is higher than the control group, experimental group IGF-1 has the significance difference in the 2-8th week-long relative expression quantity and the control group comparison (p<0.05), the 1st week lowest, expression quantity for 1.302±0.058; Achieves in the 4th week high, expression quantity for 3.914±0.273; Effective control group IGF-1 has the significance difference in the 2-8th week-long relative expression quantity and the control group comparison (p<0.05), the 1st week lowest, expression quantity for 1.278±0.074; Achieves in the 4th week high, expression quantity for 3.286±0.181. Effective control group IGF-1 must be lower than the experimental group in 1-8 week-long relative expression quantity, has statistics difference in the 4th week-long expression quantity both comparison (p<0.05). Conclusion:"the profit kidney healthy bone pellet" in can affect insulin growth factor-1 obviously from the body bone repair bone damage process in (IGF-1) the expression, accelerates the bone damage the repair and the bone damage area again vascularizing process, for clinical provides the essential science the theory basis.