| ObjectiveTo observe the effect of 'shuang gu yi tong' acupuncture therapy on OPG/RANKL/RANK in the rats of postmenopausal osteoporosis (PMO), investigate the method and working mechanism, and obtain the new way of acupuncture therapy and the Experiment basis.Methods:Experiment methods:Fifty SD rats were randomly divided into five groups:control group, model group, common acupuncture group (abbreviated as common group),'shuang gu yi tong' acupuncture group (abbreviated as shuang gu yi tong group), Herb group. The rats of model group, common group, shuang gu yi tong group, Herb group were ovariectomized to be the PMO Animal model. After 90 days, common groupand shuang gu yi tong group were treated with Electroacupuncture(Qd), Herb group was treated with Xianlinggubao (0.4g, Bid). Control group and model group were treated wi th NS. The course of treatment was 8 weeks. Electroacupuncture was treated everyday for 15 minutes. Pishu, Weishu, Shenshu, Qihaishu (double) were treated for common group; Guanyuan, Housanli, Shenshu, Geshu, Dazhu were treated for Shuang gu yi tong group. After the experiment, we detected ALP, E2, IL-6, BGP, Ca, P, M-CSF, OPG, RANKL, RANK in the blood and Ca, P, Ca/Cr, DPD in the urine; We detected BMD; We detected M-CSF, OPG, RANKL-mRNA and OPG/RANKL with RT-PCR.Results1 The level of Ca in the blood of model group was decreased, P, ALP and BGP was increased. There was significant difference between model group and control group (P<0.01). The level of Ca in the blood of Shuang gu yi tong group was increased, P, ALP and BGP was decreased. Shuang gu yi tong group was better than common group.The level of Ca/Cr, P/Cr in the urine of model group was increased. There was significant difference between model group and control group(P<0.01). The level of Ca/Cr, P/Cr was decreased. There were no significant difference between Shuang gu yi tong group, herb group and common group (P>0.05).The level of DPD in the urine of model group was increased. There was significant difference between model group and control group (P<0.05). The level of DPD of was decreased. There was significant difference between model group and Shuang gu yi tong group (P<0.01).There was no significant difference between Shuang gu yi tong group, herb group and common group (P>0.05).2 The BMD of lumbar and femur was decreased in model group. There was significant difference between model group and all treatment groups (P<0.01, P<0.05). The BMD in all treatment groups was increased. There was significant difference between Shuang gu yi tong group and other treatment groups on lumbar (P<0.05). There was no significant difference between Shuang gu yi tong group and other treatment groups on femur (P> 0.05) 3 To observe the morphological structure with light microscope. The bone trabe cula of model group was raritas, trifling. There was many breakpoint.The dist was wide. The bone trabe cula of all treatment groups was mult, thickening. The breakpoint and blank was improved. Shuang gu yi tong group was better than common group and herb group.4 The level of IL-6 was increased, E2 was decreased in model group. There was significant difference between model group and control group (P<0.01). The level of IL-6 in all treatment groups was decreased, E2 was increased. There was significant difference between model group and control group (P<0.01). Shuang gu yi tong group was better than common group and herb group.5 The level of M-CSF in the blood was increased, OPG was decreased, RANKL was decreased in model group. There was significant difference between model group and control group (P<0.01). The level of M-CSF in all treatment groups was decreased, OPG was increased, RANK was increased. There were significant difference between herb group, common group and model group(P<0.01). Shuang gu yi tong group was better than common group and herb group.6 The expression of M-CSFmRNA in the bone of model group was increased (P<0.01). The expression of M-CSFmRNA in all treatment groups was decreased. There was significant difference between common group and Shuang gu yi tong group (P<0.05). There was significant difference between herb group and Shuang gu yi tong group (P<0.01). Shuang gu yi tong group was better than common group and herb group.The expression of OPGmRNA in the bone of model group was decreased. There was significant difference between model group and control group (P<0.01). The expression of OPGmRNA in all treatment groups was increased. There were significant difference between herb group, common group and model group (P<0.01). Shuang gu yi tong group was better than common group and herb group.The expression of RANKLmRNA in the bone of model group was decreased. There was significant difference between model group and control group (P<0.01).The expression of RANKLmRNA of Shuang gu yi tong group was increased. There was significant difference between model group and Shuang gu yi tong group (P<0.01). Shuang gu yi tong group was better than common group and Herb group.The expression of OPG/RANKL in the bone of model group was decreased. There was significant difference between model group and control group (P<0.05).The expression of RANKLmRNA of Shuang gu yi tong group was increased. There was significant difference between model group and Shuang gu yi tong group (P<0.01). Shuang gu yi tong group was better than common group and herb group.Conclusion:1 Shuang gu yi tong acupuncture therapy could increase BGP, Ca, increase P, Ca/Cr, P/Cr, DPD in the Urine to regulate Ca, P metabolism.2 Shuang gu yi tong acupuncture therapy could increase E2, decrease IL-6 to increase BMD, to improve the morphological structure.3 Shuang gu yi tong acupuncture therapy could increase OPG, RANKL, RANK, decrease M-CSF to inhibit the activity of OC, to reduce the loss of bone mass.4 Shuang gu yi tong acupuncture therapy could increase the expression of OPGmRNA, RANKLmRNA, OPG/RANKL, decrease M-CSFmRNA, increase the activity of osteoblasts, prevent bone resorption.
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