Yishen Transfer Gas Research, Mental Symptoms Group The Neural Plasticity Intervention Role Of Cerebrovascular Disease

Objective:Through observing three aspects of MCI,PSD and VD rats:the evolutionary tendency of the group spirit symptoms,microcosmic structure changes of Papez loop,nerve repair and regeneration of NTF,we try to clarify the pathological and biological bases of psychiatric symptoms after cerebrovascular diseases.Using Yinao Jieyu prescription on MCI rats to observe the material foundation and mechanism in order to enrich the basic theory of TCM and the contents of Chinese pharmacology.Through this study,we hope it can provides a new treatment for cerebrovascular disease symptoms.Methods:Male wistar rats were randomly divided into normal group,sham-operation group,MCI group,VD group,PSD group,TCM(troditional chinese medicine) group and western medicine group according to the scores of open-field test.The normal group received no intervention.The sham-operation group received sham operation,and intragastric administration of distilled water.Those rats in other groups were duplicated MCI model first.The treatment group and control group received intragastric administration of Yinao Jieyu prescription and Nimodipineand respectively.All of rats in 1,2,4 weeks were observed characterizations including weight,food intake,sucrose water consumption,open-field test,jumping stair and shuttle box experiment.Then we observed ultrastructural changes of Papez loop through light and electron microscope.we also observed the BDNF,VEGF,bFGF expression changes through SABC immunohistochemistry test of brain.Results:1 The group spirit symptoms observation1.1 Behavioral evaluation1.1.1 Sucrose water consumption:The sucrose water consumption of model rats declined on 1.2.2 weeks,and significantly lower than the normal,sham-operation rats(p<0.01).The sucrose water consumption of VD and PSD rats were more lower than MCI rats,and PSD rats dropped apparently.TCM rats'sucrose water consumption were higher than model groups,but lower than the normal.2 dyeing observation:MCI rats could be observed ischemic changes,necrotic areas,cell lossed seriously,cytopathy,vascular dilatation and congestion of MCI rats in the left brain could be observed.And by observing three positions in the same mouse,we found that the hippocampus structure were destroyed obviously than hypothalamus and amygdala. 3 NTF and vascular regeneration factor determination3.1 GAP-43 determination:The expression of BDNF in the hippocampus, hypotha-lamus and hippocampus,hypothalamus and amygdala decreased in 1,2,4 weeks;MCI rats had higher expression than PSD and VD rats,but PSD and VD rats had no obvious difference between them.TCM rats'BDNF expression could maintain at the normal level.3.2Nogo-A and NgR determination:MCI,VD and PSD rats had a lower Nogo-A, NgR expression than normal rats.MCI rats in 1 week had a higher expression than the other two time position,and had no obvious difference with norml rats in 4 week.VD and PSD group of Nogo-A, NgR expression were compared to MCI group.TCM rats had a significantly higher expression of Nogo-A and NgR in 1,2,4 weeks than normal and MCI rats(p<0.01).Conclusions:1 MCI,PSD and VD,the three kinds of model rats had a series mental symptoms group changes:interest and pleasure lacking,active movement reducing,learning and memory ability droping and so on.2 Yinao Jieyu prescription can improve the changes.And it shows that early intervention with Chinese medicine can prevent the evolution of post stroke.3 The model of rats'Papez loop morphologic changed,which hinted at the internal mechanism at the evolution course of groups of mental symptoms after stroke disease for Papez loop.And Yinao Jieyu prescription may mitigate such already cerebral pathologic changes.4 The model rats exists abnormal expression of GAP-43, Nogo-A and NgR in Papez loop.So raising such factors'expression maybe one of the link in the role of evolution intervention.