The Study Of The Effect Mechanism Of Anti-airway Inflammation Of Asthma In Children With Phlegm And Blood Stasis Sgndrome Of Longli Pinchuan Tang

Objective:To reveal the scientific connotation of phlegm and blood stasis stagnating lung,treating lung and nose disease simultaneously in the pediatric asthma and allergic rhinitis, investigate the effect mechanism of anti-airway inflammation of asthma in children with phlegm and blood stasis syndrome of Longli Pinchuan Tang.Research methods:1.Group division:72mice,48rats were respectively divided into blank group, model group,dexamethasone group and Longli Pinchuan Tang high,medium and low dose group by weight and gender, with12mice,8rats each group.2.Modeling:Blank group was intraperitoneal injected and atomized excitation with saline water, other groups were sensitized by injecting the fresh albumin and aluminum hydroxide solution and atomized excitation with OVA as allergen.3. Medication method:The dose of Longli Pinchuan Tang high,medium and low of the mice and rats were respectively105.6g·kg·d,52.8g-kg-d.26.4g-kg-d and74.8g·kg·d,37.4g·kg·d,18.7g·kg·d,the dose of dexamethasone of the mice and rats were respectively3mg-kg-d,1mg·kg-d, ig capacity were respectively20ml/kg和15ml/kg,for one week.Result:1,we successfully replicated the models of asthma with phlegm and blood stasis syndrome with OVA as allergen.2,Rhinitis mucosa and lung tissue had the common inflammatory reaction mechanism and the similar pathologic morphological changes between the nasal mucosa and lung tissue of asthmatic rat.3,a large number of inflammatory cells exudation and infiltration in the lung tissues of model group,the bronchial wall structure was destroyed, epithelium shedding, alveolar wall capillary dilation congestion;4,Longli Pinchuan Tang effectively controled allergic rhinitis and asthma symptoms, reduce peripheral blood EOS concentration,significantly prolonged the latent period of asthma;5,Longli Pinchuan Tang effectively improved the expression of FasmRNA, reducing the expression of Bcl-2mRNA, induced apoptosis of inflammation cells, significantly up-regulate IFN-y level,downregulate IL-4level,reverse IFN-γ/IL-4ratio imbalance, rapidly correct abnormal elevation of the whole blood viscosity and hematocrit of asthmatic rats. Conclusion:1,The results demonstrated the scientific nature of the same pathological changes of both lung and nose,treating lung and nose disease simultaneously in the pediatric asthma and allergic rhinitis;2,The study suggested that the main pathological mechanisms of children in asthma in onset stage were invasion of exogenous pathogens,the same pathological changes of both lung and nose,phlegm and blood stasis stagnating lung, expounded that phlegm and blood stasis stagnating lung and its the inherent essence relationship between phlegm and blood stasis stagnating lung and airway inflammation, revealed part of the scientific connotation of phlegm and blood stasis stagnating lung;3,The effect mechanism of anti-airway inflammation of asthma in children with phlegm and blood stasis syndrome of Longli Pinchuan Tang was related to promoting inflammation cell apoptosis,reversing IFN-γ/IL-4ratio imbalance, correcting blood circulation disorders.