Research On The Relationship Between ORP9Gene Polymorphisms And Cerebral Infarction

Background and ObjeetivesOxysterol-binding protein-related protein9(ORP9) was a kind of sterol transporter which was related with atherosclerosis,lipids and glucose.There was no reports about the gene polymorphism of ORP9home and abroad. We are the first to investigate the genotypic and allelic distributions of ORP9in Hunan Han population,and first analysis the association of the ORP9gene polymorphisms with cerebral infarction, hypertension, the metabolism of the blood lipid and blood sugar and the formation of carotid atherosclerosis.MethodsIn this study,we choose the subjects from the Hunan Han population that had been to Xiangya Hospital of Central South University. Atherothrombosis cerebral infarction and Small Artery Disease patients were chosen as CI group,age-and sex-matched healthy individuals were chosen as the control group.There were no phylogenetic relationships among the subjects.We asked the case history and recorded the examination results seriously.We collected fasting venous blood from every subject early in the moring and obtained DNA from the blood samples using Phenol/chloroform method.By using PCR-RFLP method, we detected the genotypic and allelic distributions of rs856600and rs768529and analysis the association of the polymorphisms with cerebral infarction, hypertension, the metabolism of the blood lipid and blood sugar and the formation of carotid atherosclerosis.For rs856600,objects studied were composed of406individuals totally:(1)control group was made up of94males and57females,totally151individuals,with the mean age59±9.5years;(2)CI group was composed of167males and88females,totally255individuals,with the mean age60±10.29years.For rs768529, objects studied were composed of406individuals totally:(1)control group was made up of88males and45females,totally133individuals,with the mean age58±10.75years;(2)CI group was composed of193males and96females,totally289individuals,with the mean age60±10.80years.Results1. The genotypic and allelic distributions of rs856600and rs768529had no significant differences between the CI group and the control group,the AT,SAD group and the control group (P>0.05).Logistic rergression analysis showed that both the genotypes and the alleles frequency could not increase the risk of the onset of CI(P>0.05).2. The genotypic and allelic distributions of rs856600and rs768529in the CI group and the control group had no significant differences between the hypertension subgroup and the non-hypertension subgroup,either between the diabetes supgroup and the non-diabetes group(P>0.05).3. In the control group and the CI group,the hypertention,the serum level of FBS,TG,TC,HDL and LDL of had no significant differences between the T allele(GC+CC) carriers and the TT genetype carriers for rs856600,either between the T allele(GC+CC) carriers and the TT genetype carriers for rs768529.4. The genotypic and allelic distributions of rs856600had no significant differences between the WAS and AS,the SP and VP subgroup (p>0.05); The genotypic and allelic distributions of rs768529had no significant differences between the WAS and AS subgroup (p>0.05),but the frequency of T allele is higher of the VP subgroup than the SP subgroup (p<0.05) in the CI group.Conclusions1.Association of the rs856600and rs768529Polymorphisms with cerebral infarction in Hunan Han Population may not exist.2. T allele of rs768529may be a risk factor for the formation of the carotid vulnerable plaque in Hunan Han population.3. Association of the rs856600polymorphisms and the formation of the carotid vulnerable plaque in Hunan Han population may not exist;4.Association of rs856600and rs768529polymophisms with the occurrence of hypertention and the metabolism of blood lipid and blood sugar in the CI and healty Hunan Han population may not exist.