Study Of The Inhibition Effects And Mechanism Of Cur-PLGA-NPs On Hela Cells

OBJECTIVE:1.To prepare curcumin-loaded PLGA nanoparticles (Cur-PLGA-NPs) and to investigate the inhibition effects of Cur-PALG-NPs on the proliferation of Hela cells.2.Preliminary study the mechanism of the inhibition effects.METHODS:1.Cur-PLGA-NPs were Synthesized by modified emulsion-solvent method. The particle size and morphology were validated under transmission electron microscopy. The drug loading was detected by the UV-vis spectrophotometry.2.Cultivating human cervical carcinoma Hela cells in vitro, the inhibition effects of Cur-PLGA-NPs on Hela cells were observed with MTT assay. The apoptosis rate and the distribution of cell cycle were detected by FCM respectively.3.Intervening Hela cells with different concentration of Cur-PLGA-NPs, the differences of HPV18E6, E7RNA content in cells due to effects of Cur-PLGA-NPs were detected by fluorescence quantitative PCR and the the differences of E6, E7proteins expression by western blot method.RESULTS:1.The preraration of Cur-PLGA-NPs was successful and the sizes of spherical nanoparticles were mainly distributed in the30nm-150nm range. The drug loading was4.6%.2.6.25μumol·L-1,12.5μmol·L-1,25umol·L-1,50μmol·L-1Cur-PLGA-NPs inhibited the proliferation of Hela cells significantly(P<0.01), with dose and time dependent, and resulted in cell apoptosis(P<0.01). In the same concentration, Cur-PLGA-NPs played stronger role of the inhibition effects than curcumin(P<0.01).50μmol·L-1Cur-PLGA-NPs arrested the cells in G1phase obviously(P<0.01).3. After intervening of Cur-PLGA-NPs in different concentrations, the HPV18E6, E7RNA decreased(P<0.05), and the decreasing sevenrity was related with drug concentration.4.After intervening of different concentrations of Cur-PLGA-NPs, the inhibition effects on E6, E7protein expression were related with drug concentrations(P<0.05).CONCLUSION:1.Cur-PLGA-NPs with superior traits were synthesized with optimal emulsion-solvent preparition method, and inhibited the proliferation of Hela cells significantly.2.The inhibition mechanism of Cur-PLGA-NPs may be down-regulation of the HPV18E6, E7RNA and the inhibition of the E6, E7proteins expression.