The Impact Of Power For The Signal Transduction Mechanisms In Hypertensive Intracerebral Hemorrhage In Rat Hippocampus

Objective Cerebral hemorrhage is a common disease, which threatens the human's health and life with high disablement and fatality rate. Acupuncture, which has a long therapeutic history of cerebral hemorrhage, brings a significant therapeutic effect on it and has vast vistas. Based on the clinical treatment of cerebral hemorrhage with Shuigou, Neiguan and Zusanli acupoints, we studied the therapeutic effect and mechanisms of electroacupuncture on the rats suffered with hypertensive cerebral hemorrhage hi order to apply theoretical and experimental bases for the clinical practice.Methods On the base of renovascular hypertensive rats (RHR) model, the hypertensive cerebral hemorrhage rat model was induced by injecting collagenase and heparin into the brain. The therapeutic effect on hypertension, nerve missing sign, pathologic change and the content or expression of Gs a, Gi a, Go a, Gi a mRNA, cAMP, SS, SSmRNA of the rats with hypertensive cerebral hemorrhage on different phase after electroacupuncture were observed and compared with those of the normal group, false operation group and model group.Results (1)The hypertension and the nerve missing sign of the rats were significantly improved by electroacupuncture; (2) Electroacupuncture may significantly improve the pathologic change in neuron of hippocampus and protect the neuron; (3) The content of Gi a and Go a hi hippocampus of model group rats were significantly lower than those of normal and false operation group rats, and the change of Gi a is obvious. While after electroacupuncture, they were significantly increased with the lapse of time. But the content of Gs a has no significantly change hi the model group rats; (4) The expression of Gi2 a mRNA and GiaCxmRNA of model group rats were significantly weaker than those of normal and false operation group rats, while after electroacupuncture, they were significantly increased with the lapse of time; (5) The content of cAMP hi hippocampus of model group rats were significantly higher than those of normal and false operation group, while after electroacupuncture, they were significantly decreased with the lapse of time; (6) The expression of SS and SSmRNA hi hippocampus of model group rats were significantly weaker than those of normal and false operation group rats, while after electroacupuncture, they were significantly increased with the lapse of time.Conclusion The hypertensive cerebral hemorrhage rat model which is based on RHR and induced by injecting collagenase and heparin into the brain is similar hi physiology and pathology to human hypertensive cerebral hemorrhage, the common type of cerebral hemorrhage clinically. The pathological changes of Gi a, Go a, Gi a mRNA, cAMP, SS and SSmRNA in hippocampus of hypertensive cerebral hemorrhage model rats indicate that there is the disorder of signal transduction in neuroendocrine system. Electroacupuncture on Shuigou, Neiguan and Zusanli maynot only control the hypertension and improve the nerve missing sign, but also significantly adjust the pathological change of the indexes mentioned above, which indicates that electroacupuncture may adjust the signal transduction system with polylink and polytarget of first massenger, G protein hi transmembrane signal transduction and second messenger, so as to prevent the pathological progress and improve the prognosis of cerebral hemorrhage.