| Objective Hypothyroidism is a common, difficult and complicated disease of endocrine system, which is a syndrome of hypometabolism caused by the deficiency of the synthesis, secretion and biological effect of thyroid hormone. Till now, there are no radical therapeutic methods for it in clinic. Chinese medicine may bring significant therapeutic effect on it. To take vigorous action on searching for a new therapeutic method for prevention and treatment of hypothyroidism by giving full play to the advantages of Traditional Chinese Medicine such as whole adjustment, less side effect, and long-term therapeutic effective, on the base of the clinical practice with Banliuwan, we study the function of Banliuwan on hypothyroidism and its mechanism under the basic theory and experimental methods of modern medicine with the hypothyroidism animal model in order to provide a theory and experimental base for the clinical practice.Methods The rats were randomly divided into 6 groups, such as normal control group, model group, thyroxin group, small dosage group of Banliuwan, large dosage of Banliuwan, and the integrated group of thyroxin and Banliuwan. The rats of the latter 5 groups were made model by infusing 0.04% tapazole suspension 1.35ml/200g/day for 45 days. After that, the rats were treated with corresponding medicines for 15 days. Then the following detection were taken out: radioimmunoassay on serum FT3, FT4, T3, T4, TSH and sexual hormones (FSH, LH, E2, P, T); RT-PCR, Western blotting, Northern blotting and immunohistochemical method on the content or express of T3NRmRNA, Gsα, Gi α, Go α, Goα mRNA and SS; optical microscope and/or electron microscope observation on the pathology of thyroid and genitals.Results (1) In the rats of model group, the behavior changes of kidney yang-insufficiency in hypothyroidism were appeared, and the indexes which may imply the severe degree of hypothyroidism presented the decreasing of FT3, FT4, T3 and T4, and the increasing of TSH, at the same time, the thyroid had retrograte changes in pathology. Banliuwan, especially with large dosage had not only improved the symptoms of hypothyroidism, but also increased the content of FT3, FT4, T3 and T4, and decreased the content of TSH, at the same time, it had improved the retrograte changes of the thyroid; (2) In the rats of model group, the level of serum sexual hormones (FSH, LH, E2, P, T) were obviously decreased and the genitals presented retrograte changes pathologically. Banliuwan significantly increased the content of sexual hormones and improved the structure of genitals. The effect depended on the dosage, that is, the effect of large dosage group was better than that of small dosage group. (3) In hippocampus of the hypothyroidism rats, there were decreased expression of T3NRmRNA, increased expression or content of Gi α, Go α, Go α mRNA and SS. Different dosage ofBanliuwan brought different adjustment function on them, among which the best therapeutic effect was brought in large dosage group.Conclusion Banliuwan, which may inspire yang-qi to promote the functional activity of the body, has good therapeutic effect on hypothyroidism rats with the improvement of symptoms, increasing of thyroid hormone and sexual hormone, improvement of the retrograte change of genitals and thyroid, as well as the adjustment of the pathological change of TaNRmRNA, Gi a , Go a, Go a mRNA and SS in hippocampus. The mechanism of Banliuwan on the symtom and the polysystem disorders of hypothyroidism, such as genital system and nervous system dysfunction, may be that Banliuwan may increase the endogenous thyroid hormone, reduce the injury by the damaged signal transduction, and adjust the neuroendocrine disorders. |
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