| Zhong Jiling is an effective herbal prescription to treat Myasthenia gravis (MG) mainly consisting in Radix Astragali Seu Hedysari, Radix Ginseng and Hairy Antler etc.. Zhong Jiling has been used to treat more than thousands MG cases and got good result. MG is an autoimmune disease, which mainly involves the antibody of acetylcholine receptor, located on posterior membrane of synapse at the junction of nerve-muscle and is conducted by acetylcholine receptor and relys on T-cell and complements join it. This thesis, which establishing EAMG model in rats and focusing on thymus-Th subgroup-IL4, IFN-r-anti-N2AchR antibody and N2AchR etc., studied the functional mechanism of Zhong Jiling preventing from MG from different level such as whole, tissue, cell and molecule. The main study contents are as below:1. Establishment of EAMG model in rats First, extracted rough N2AchR from crassinarke dormitory Takagi electric organ and mixed it with CFA in the same amount, and then immunized for 3 times by multiple injecting antigen liquid contained N2AchR 0.5 ml/rat on tails, feet and backs of rats. The valuation of EAMG model is from clinical symptoms, weight, muscular force, muscular motion ability, Electromusculagram, loss rate of N2AchR in muscle and N2AchR titre in blood serum. The results show that, comparing with normal group and CFA group, the above indexes in model group have significant differences (p<0.01), which is the EAMG model is established successfully.2. Study on pharmacodynamics of Zhong Jiling in EAMG rats This is to observe the prevention effect of Zhong Jiling to EAMG rats. The results are as below: Zhong Jling in big and medium amount (6.56g/kg, 3.28g/kg) notably improved the clinical symptoms of EAMG rats (p<0.01 and p<0.05) and increased weights in EAMG group; the study of rat climbing-net shows that Zhong Jiling in big and medium amount can notably improve muscular force of EAMG rats (p<0.05); the results of the study on motion ability of rats were that Zhong Jiling in big and medium amount increased rat's climbing net numbers and combing hair numbers (p<0.01 and p<0.05), it shows that Zhong Jiling can remarkably improve motion function of EAMG; Zhong Jiling in big, medium and small amount all reduced the level of anti- N2AchR-antibody in blood serum of EAMG rats(p<0.01 and p<0.05); Zhong Jiling in big and medium amount raised the contraction decline of rat musculus gastrocnemius after stimulating sciatic nerves of EAMG rat; Zhong Jiling in big, medium and small amount all increased the N2AchR numbers on posterior membrane of synapse of posterior leg muscle of EAMG rats(p<0.01 and p<0.05) and the receptor numbers are respectively 78%, 60% and 48.7% of normal group( model group id 31%).The above results show that Zhong Jiling has a significant function of EAMG prevention and treatment.3. Effects on immune function of Zhong Jiling to EAMG rats and normal mice 3.1 Effects on immune function of Zhong Jiling to EAMG rats Zhong Jiling in big, medium and small amount obviously enhanced T-cell proliferation of lymphocyte of EAMG rats induced by ConA (p<0.01 and P<0.05), and dexamethasone (DXM) restrained its proliferation (p<0.01); Zhong Jiling in big, medium and small amount inhibited T-cell proliferation of lymphocyte of EAMG rats induced by specificity antigen of purified N2AchR (p<0.01) and DTH reaction (p<0.01), and DXM has the same above effects as zhong Jiling.3.2 Effects on immune function of Zhong Jiling to normal mice Zhong iling in big and medium amount enhanced T-cell proliferation of spleen of normal mice induced by ConA (p<0.01); Zhong Jiling in medium and small amount improved cells which produce antibody and increased the antibody titre of blood coagulation (p<0.01).The above results show that Zhong Jiling can significantly inhibit cellular immunoreaction and humoral immunoreaction of EAMG rat induced by specificity antigen of N2AchR, but zhong jiling enhances cellular immunoreaction induced by ConA and humoral immunoreaction induced by SRBC. This has proved t...
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