| This paper is composed of three parts.They are theoretical study,inherited experiences from tutor,and experimental study.The first part of theoretical study is consisted of three reviews which are related to the contents of experimental study, including the state of study on insulin resistance of traditional Chinese medicine(TCM),the relationship between lipodystrophy and type two diabetes mellitus , the relationship between tumor necrosis factor α and insulin resistance.Review one includes the relationship between insulin resistance and the pathomechanism of TCM or pattern identification which belongs to type two diabetes mellitus,hypertension and metabolic syndrome and the progress and problem of research on the improvement of insulin resistance by TCM. Review two is mainly about the progress of research on obesity and lipodystrophy, lipodystrophy and insulin resistance,the toxicity which caused by lipodystrophy on pancreatic β cells. Review three is mainly about the progress of research on the relativity of tumor necrosis factor α and insulin resistance,the mechanism or the pathway or the treatment of insulin resistance which caused by tumor necrosis factor α.The second part of inherited experiences from tutor discusses the academic theory of the toxin of tutor,viewpoint of tutor that the toxin which is caused by vacuity of Pi is the pathomechanism and principle of treament of Xiaoke disease.The third part of experimental study used method of injection a low dose of strepozotocin through vein of rat's tail followed by a high caloric diet to duplicate rat model of insulin resistance of type two diabetes mellitus successfully and divided experimental rats into the control group,model group,TangKe decoction group and rosiglitazone group,measured the influence of TangKe decoction on insulin resistance by insulin-sensitivity index,explored the mechanism from the influences of TangKe decoction on lipodystrophy, tumor necrosis factor α which was produced by lipid and insulin signal transduction.Experiment one detected the blood lipid content,blood free fatty acid content and blood nitric oxide content of experimental rats.Experiment two detected tumor necrosis factor α expression in adipose tissue of experimental rats and expression level of glucose transporter 4 in skeletal muscule of experimental rats. Experiment three detected the protein tyrosine kinase activity of insulin receptor which was purified of skeletal muscule cell and the tyrosine phosphorylation degree of insulin receptor β subunit of experimental rats,observed glucose transporter 4 translocation of skeletal muscule cell of experimental rats.The result showed that TangKe decoction could increase the insulin sensitivity of rat model of insulin resistance of type two diabetes mellitus by improving its lipodystrophy,especially decreasing its levels of blood free fatty acid and blood triglyceride,increasing its level of blood nitric oxide and improving its vascular endothelium cell function,decreasing level of tumor necrosis factor α expression in adipose tissue, increasing its protein tyrosine kinase activity of insulin receptor which was purified of skeletal muscule celland its tyrosine phosphorylation degree of insulin receptor β subunit,increasing its glucose transporter 4 translocation of skeletal muscule cell,but the protein content of glucose transporter 4 of skeletal muscule cell was unchanged probably.
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