Shao Gan The Polyglucosides Liver Protective Effect And Mechanism

Using the ancient famous prescription Shao Yao Gan Cao Tang as the foundation ,we get the effective clusters of Shao Yao Gan Cao Tang -Shao Gan Duo Gan through extraction,separation and purification. It's a new effective drug for curing chronic hepatitis B. Based on the theory of traditional Chinese medicine and the current achievements of Modern Medicine, combined the characteristics of the drug , refered to the state guideline of pharmacodynamic action about anti-hepatitis, this study investigate Shao Gan Duo Gan's main effect of liver protection using acute liver injury induced in mice by carbon tetrachloride and D-galactosamine, subacute liver injury induced in rat by carbon tetrachloride, chronic liver injury induced in rat by carbon tetrachloride and immunological liver injury induced by lipopolysaecharide in bacillus calmette guerin prime mice. At the same time ,by means of modern technology such as micorscoope, electric mi.coroscope, computer image analysis system, RIA , gene chip etc, we systematically investigate the liver protective mechanism of Shao Gan Duo Gan, and discuss the molecular mechanism of it at genome level.The experiments show that Shao Gan Duo Gan has an obvious effect of liver protection, can remarkably reduce the transaminase, alleviate the pathologic change of acute and chronic liver injury in mice .or rat, and can alleviate hyperplasia of collagen fibers, prevent liver cirrhosis in rat with chronic liver injury.The experimental results prompt that the likely mechanism of liver protection about Shao Gan Duo Gan are (1) antioxidation, can inhance the activity of SOD, reduce the content of MDA in liver and mitochondria; (2)reduce the contentof TNF , IL-1 , IL-8 in liver , alleviate the cytokine injury to liver; (3)reduce the activities of total NOS, iNOS and the content of NO; (4)reduce the content of TXA2, inhance the ratio of PGI2/TXA2; (5)protect the ultrastructure of liver, make the structure of organelle for example mitochondria,endoplasmic reticulum to normal, hepatin to abundance;(6)alleviate the injury of mitochondria,can obviously alleviate swell and denaturalization of mitochondria, inhance the ability of antioxidation of mitochondria , the metabolism of energy and the activity of calcium pump, preventthe calcium over loading and dropsy of mitochondria;(7)the gene chip results show that Shao Gan Duo Gan's regulations to the gene of liver are multitarget,this multichannel changes of target genes are likely to be the molecular mechanism of liver protection, embody in (1)up-regulate the gene expression about energy motabilsm ; (2) up-regulate the gene expression of protein and enzyme about transcribe ,cell proliferation and cell cycle; (3) down-regulate the gene expression about apoptosis; (4)down-regulate the gene expression about liver fibrosis; (5)up-rgulate the gene expression about hyperirritability;(6)regulate the gene expression about metabolic enzyme of liver; (7)regulate the gene expre- ssion about immunity andinflammation;(8)up-regulate the gene expression about antioxidation;(9)up-regulate the gene expression about transfer.