Pass Through The Back Of Milk To The Treatment Of Hyperprolactinemia Compound Optimization

To study and develop a new Chinese patent compound formula on treatmentof hyperprolactinemia and explore a method in reseaching new drugs of moderndisease, six steps were carried out in the test: the design of prescription,the animal experiment of testing the prescription, the clinical experimentof testing the prescription, the further optimization of prescription, theanimal experiment of testing the optimum prescription and the clinicalexperiment of testing the optimum prescription. The key to the study is the design of prescription. There are threeprinciples need to be obeyed in the design of prescription: differentiationof syndromes, identifying pharmacodynamics and following the EBM in usingTraditional Chinese medicine. We designed a prescription called TongjingHuiru Recipe (THR), which is made of seven Chinese medicinal herbs. The animal experiment was carried out to test the effect of theprescription. After successfully duplicating the animal model ofhyperprolactinemia, 50 rats were randomly divied into 5 groups and treatedwith THR. The level of serum prolactin(PRL), esterovite(E2) andprogesterone(P) were determined and compared with the blank control groupafter treatment. It was observed that THR could decrease the level of serumPRL and increase the level of serum E2 and P effectively. Then, 30 patients of hyperprolactinemia were treated with THR to testthe clinical effect of THR. Radioimmunossay was used to determine levels ofserum PRL, luteinizint hormone, follicle-stimulating hormone, E2 and P beforeand after teratment. THR could decrease the level of serum PRL and increaselevels of serum follicle-stimulating hormone and E2 safely and effectively.6 通经回乳方治疗高泌乳素血症的复方优化研究 To find the optimum prescription of THR, we did the animal experimentof the further optimization of prescription. 110 rats were randomly diviedinto 11 groups and treated with THR after successfully duplicating the animalmodel of hyperprolactinemia. The level of serum prolactin(PRL), esterovite(E2)and progesterone(P) were determined and compared with the blank control groupafter treatment. It included seven steps which is definition of dose of allmedicine in the prescription, drowing of factor-level chart, experimentarrangement by RDZ, proceed of pharmacodynamics, solution of regressionequation by SAS , solution of the optimum prescription in different effectmarker and integration of the optimum prescription. The optimum prescription,which is made of five traditional Chinese herbs, was concluded with theresulting model. The fifth part is to test the optimum prescription and explore itsmechanism. 60 rats were randomly divided into 11 groups and treated with theoptimum prescription after successfully duplicating the animal model ofhyperprolactinemia. The level of serum prolactin(PRL),esterovite(E2) andprogesterone(P) were determined and compared with the blank control groupafter treatment. It was observed that the optimum prescription could decreaselevels of serum and pituitary gland PRL, increase the level of serum E2 andP ,further reduce PRL mRNA expression significently,promote folliculardevelopment and oestrus change of mucous membrane ,inhibit hyperplasia ofmammary gland in the pathology and histology. Another clinical trail of 30 cases of hyperprolactinemia which treatedwith the optimum prescription was conducted. Radioimmunossay was used todetermine levels of serum PRL, luteinizint hormone, follicle-stimulating英文摘要 7hormone, E2 and P before and after teratment. The optimum prescription coulddecrease the level of serum PRL and increase levels of serumfollicle-stimulating hormone and E2 safely and effectively. Through studying a new Chinese patent compound formulaon treatment ofhyperprolactinemia, we exploring a method of researching new drugs on moderndisease.