Heat Of Wet Experimental Study Of Febrile Disease And Its Mechanism Of Damp-heat Syndrome Model

Objective: 1. To establish models of damp-heat syndrome rats by compound factors, on the basis of arranging and analyzing on the damp-heat syndrome in the ancient and modern literatures. 2.To set hypothesis which are "the abnormality of lipid is one biochemical foundation of damp-pathogen" and "epidemic febrile disease syndrome of damp and heat is relating to the gastrointestinal motility", on which to observe the effect of clearing away heat and dampness (compound therapy). Meanwhile, take comparative research among the method of clearing away heat and relieve toxicity (fraction therapy) and the method of dispersing Qi to remove dampness (fraction therapy), and explain the mechanism from the molecule level of curing rats damp-heat syndrome models' gastrointestinal motive disorder, moreover regulate the effect and mechanism of blood lipid's metabolic abnormality, and discuss the method of clearing away dampness and heat factors, especially clearing away dampness' sector and target. On this basis interpret scientific connotation of making damp and heat leave each other and be removed to cure damp-heat syndrome.Methods: The theoretics study adopts the methods of collection, arrangement and analysis, to sum up the ancient scholars' knowledgement, differentiation and the characters of cleaning away damp and heat method, to statistically analyse the clinical and experimental literature in modern times, view clearly the basic phenomenon of mechanism research on clearing away heat and dampness. The experimental research discuss the clearing away damp-heat method's mechanism of clearing away dampness and renew the spleen and stomach's function as the point of the research. With rats damp-heat syndrome models, to systematically and deeply observe the effect and mechanism of the clearing away heat and damp method's curing the models gastrointestinal molive disorder, and the effect and mechanism of regulating blood lipid's metabotic abnormality, furthermore systematically and deeply research into the effect of the models' ADH. In the meanwhile, probe into the differentiation of B,C and A in the mechanism. To hope that according to the research in the above and from lots of sectors such as lipid's metabolism, gastrointestinal hormones and genetic expression, explain the method's partial mechanism of regulating epidemic febrile disease syndrome of damp and heat's gastrointestinal motive disorder and recover the transportation. Function of the spleen and stomach , to lay foundation of comprehensively interpreting this method's curing damp-heat syndrome scientific innovation.Process: To inspect A, B, C's effect on rats' epidemic febrile disease syndrome of damp and heat obstruding in the middle models, adopt a richly fatty and sweet diet, high temperature, additional infeded typhoid fever bacterium and other compound factors to imitate rats' epidemic febrile disease syndrome of damp and heat models. And randomly divided the rats into normal control group, damp-heat syndrome group. A group ,B group, C group, masculine compartive group. 1. The method of radio immunoassay was used to detect rats' blood MTL in gastric antrum and colon, GAS, SP and SS. 2.The method of immunohistochemistry was used to detect rats' SP expression level and LDL-R in liver. 3.The method of in situ hybriclization was usedto detect rats' SSRlmRNA expression level. 4. The method of enzyme was used to content of TC, TG, LDL-c, HDL-c in blood. 5.Reverse transcription enzyme chain reaction to detect LDL-RmRNA expression level. 6. The method of enzyme was used to detect rats' ADH.Results: In theoretics study, exotic damp and heat, the disorder of diet injure in spleen and stomach , stagnation of dampness and water pathogen are the epidemic febrile disease syndrome of damp and heat's major etiological factors and pathogenesis , The factors in the above leading to obstruction of damp and heat, the disorder ascent and descent activity of Qi and the disorder of transportation are the syndrome's crucial pathogenesis. In order to cure external damp-heat syndrome, Ye Tian...