Febrile Disease Damp-heat Syndrome Rat Model Of Experimental Study Of The Immune Parameters

ObjectiveThis experiment were based on traditional Chinese medical science theories. Adopted the combined method to establish the model of damp-heat syndrome of seasonal febrile disease and interfered with Qingre Huashi Prescription on the model. Observed the changes of tumor necrosis factor-α(TNF-α),interlerkin-1β(IL-1β) and secretary immune globulin A (SIgA). Then to provide basis for the further research of scientific examination and clinical practice by exploring some properties of damp-heat syndrome from the aspect of immunology and the act of high fat diet on the formation of damp-heat syndrome.MethodsThe damp-heat syndrome model was established by the combined method of giving high fat diet,high temperature, high humidity and escherichia coli,and interfered with Qingre Huashi Prescription. Set up normal group (A group), model group (B group), contrary group (C group) and treatment group(D group).In A and C groups the rats were raised on ordinary feed, but the rats in B and D groups were raised on high fat feed. Then to put B, C and D group into artificial climate box with the temperature of 35℃and the relative humidity of 95%. On the 26th day, to give the rats in the dose of 2g/ml Qingre Huashi Prescription every 200 gram weights three times a day in D group. On the 27th day, B, C, D group took 10~9/ml escherichia coli every 200 gram weights respectively, and after four hours repeated. The rats took 2g/ml Qingre Huashi Prescription every 200g weights three times a day for three days in D group. Continue to put the three groups into artificial climate box for three days. Observed the changes of temperature, weight, vitality activity, quantity of drinking and eating, urine, stool and hairs. On the 30th day, all of the rats were executed, and the contents of TNF-α,IL-1β,SIgA were detected by radio-immunity measures. Then the data were compared with single factor analysis of variance by using statistical method of SPSS13.0.The results were indicated by mean add-subtract standard deviation((?)±s),P<0.05 and P<0.01 means that there is statistic difference.ResultsThe symptom and signs in normal group had not any evident changes. In model group the rats occured to fever, thinness, poor appetite, tiredness and lethargy, yellow turbid urine, loose stool, dark yellow and lusterless hairs. Rats in contrary group occur to a shorter increase in temperature, anorexia, thinness, tiredness and lethargy, yellow turbid urine, pulpy stool, dark yellow and lustertess hairs. The symptom and signs in treatment group were slighter and recovering more quickly.The contents of serum TNF-α,IL-1β,SIgA in model group and contrary group were remarkably increased, and had a significant difference from the normal group (P<0.05 or P<0.01). All of them in contrary group had not any evident difference from the model group (P>0.05). The contents of serum TNF-α,IL-1β,SIgA in treatment group were decreased, and had a significant difference from the model group (P<0.05).ConclusionIn model group and contrary group the symptom and signs basically conformed to the characteristic of damp-heat syndrome. The fever in model group maintained long and was conformed to the characteristic of lingering. The contents of serum TNF-α,IL-1β,SIgA in contrary group had not any evident difference from the model group. It means that high fat diet is not a decisive factor on the formation of damp-heat syndrome.The remarkable increases of serum TNF-α,IL-1β,SIgA in model and contrary groups had extensive physiological and pathology effects, and they could well explain the law of pothogenesy and some contradictive clinical manifestations of damp-heat syndrome. The physiological and pathology effects that caused by the increase of SIgA similarly had the property of damp. Those effects caused by the increase of TNF-α,IL-1βshould have dual properties of damp and heat and that needed a further prober in after experiment. A combination of the three indicators could well explain contradictive clinical manifestations of damp-heat syndrome from immunization. The increase of SIgA also could interpret that the damp and heat were binding, the pathogenesis was slow and the course was lingering in damp-heat syndrome.Qingre Huashi Prescription could improve the symptom and signs of model rats obviously, and made the immunology parameters recover. It means that this prescription have a better reducing fever and deliminating dampness effect.