| The purpose of this study is to investigate the role and mechanism of free radicals and reactive oxygen species in retinal photic injury which was proposed to be related to the age-related macular degeneration(ARMD). Three parts included in this study were as follows:A. In order to investigate the relationship between the retinal lipid peroxidation ( LPO) or antioxidation ability and age, 22 rats of different age group were used to detect the content of retinal malondialdehyde(MDA) producted by LPO and superoxide dismutase (SOD) activity which represented the antioxidation ability of the retina with the thiobarbituric acid( TBA) and chemical luminescence methods. The results showed that the content of retinal MDA increased and SOD activity decreased with aging. The cumulative effects of the long-term photochemical damage and decreased antioxidation ability in retina were suggested to be the causes of the increased retinal LPO.B. The singlet oxygen(1O2), superoxide anion(O2-·) produced by isolated pig retinal neuroepithelia after light exposure was observed by means of chemical reaction detecting methods. The reaction showed in the process could be relatively inhibited by β -carotene, superoxide dismutase (SOD) and catalase(CAT) . The electron spin resonance (ESR) signal (g=2.0078± 0.00024) of different intensity was observed in thesamples of isolated pig's retinal neuroepithelia which were radiated by red, blue and white light in different lux. The...
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