| ObjectiveAlzheimer disease,(AD) is one of one kind of severely impair senior citizen physical and moral integrity significant diseases.Drops,the cognition function barrier take the memory to denaturate the vigorous sickness as the characteristic central nervous system.The aging of population is more serious, stupid is sick the probability to be more,the family and society's burden is also bigger.Statistics indicated that in the world 65 year-old above old age crowd approximately 10% contract the dementia.The senile dementia has substituted for the apoplexy gradually,the position first which gets sick according to the department of neurology illness.In the 21st century the humanity enters the aging of populations which faces,causes the senile dementia to become the domestic and foreign medical research an important topic.Because AD the course is long,the condition is serious,and the mid and late stage treatment result is not good treats spends in a big way.Therefore,changes the early intervention to the AD research.Acupuncture has the early time,functionality and benign bidirectional adjustment characteristic,but passes through the Chinese medicine acupuncture study "to govern has not gotten sick" the thought to be suitable for disease earlier period diagnosis and the treatment,should play the influential role in the AD prevention,but the present report prevents and controls AD about acupuncture clinical and the experimental study are very actually few.To further discuss acupuncture to the AD early prevention and the therapeutic action,this topic injects in the ventricles of the brain Aβ25-35 establishes the AD big mouse model,succeeds after the model the acupuncture and the Moxa big mouse hundred can with the kidney Yu hole.Unites the immunity adsorption through the enzyme(ELISA) to test the technology,analyzes the pre-acupuncture to free radical GSH and the LPO content examination,compared with Moxa,the piquada,the piquada + Moxa three kind of different intervention measure contrast curative effect,prevents and controls AD for acupuncture the application to provide the experiment on clinical to rest on.MethodsCarries on the Morris water labyrinth test separately 140 big mice,screens 120 big mice,the distinction divides into the normal group,the sham-operation group,the model group, the pre-Moxa group,the pre-piquada group,pre-piquada Moxa group,altogether 6 groups,each group of each 20.Will agglutinate the condition Aβ25-35 the three-dimensional direction detection to pour into the big mouse two-sided ventricles of the brain to establish the stupid big mouse model.The pre-Moxa group namely makes the mold before the big mouse to give Moxa to stimulate first,selects the big mouse "hundred meetings"(center parietal bone),"kidney Yu" (under the 2nd lumbar vertebra both sides) above the hole 2-3cm place to hang the moxibustion,causes the acupuncture point epidermis temperature in 43℃±1℃,continues 10 minutes, dailyl time,on5this 1 treatment course,the treatment course rests on 2nd,altogether 3 treatment courses.Executes the moxibustion conclusion namely to start for the big mouse brain to inject the same day Aβ25-35.The pre-piquada group namely executes first to the big mouse in the piquada stimulation.The acupoint selection with C group,hundred will puncture 3-5mm evenly forward,kidney Yu Xueshao punctures 5mm slanting inward, will join the marine product G6805 piquada treatment meter, will select the continuous wave,frequency 2Hz,take the needle handle slight vibration as,will keep needle 20min,daily 1 time, on 5th will be 1 treatment course,the treatment course rests on 2nd,altogether 3 treatment courses.The piquada conclusion namely starts for the big mouse ventricles of the brain to inject the same day Aβ25-35.The pre-piquada adds the moxibustion group namely to the big mouse first Shi gud the group and E group of same method of treatment.The treatment conclusion namely starts for the big mouse ventricles of the brain to inject the same day Aβ25-35.The Morris water labyrinth tests the big mouse study memory ability.Unites the immunity adsorption through the enzyme(ELISA) to test the technology,analyzes the pre-acupuncture to free radical GSH and the MDA content influence.Result(1) we observes from the Morris water maze experiment,each group of big mice increase along with the training number of times,the incubation period is getting more and more short, showed that lives in the big mouse respectively,in each time is swimming in the training,regarding seeks for the platform to have certain study memory ability,but its study memory ability has the very big difference.The normal group and the sham-operation group compare,the non-significance difference, showed that the surgery damages to the big mouse's study memory ability non-significance influence.In model group big mouse Morris water labyrinth incubation period 5 days maintain the constant levels basically,pass through the original platform position the number of times to be few,have the unusual significance difference with the normal group comparison, showed that the model group big mouse study memory suffers injury seriously,makes the mold to be successful.Each treatment group big mouse escape incubation period obvious reduction,passes through the original platform position the number of times to increase obviously,the swimming way also gradually reduces,and the pre-piquada adds the moxibustion group to surpass pre-Moxa the group and the pre-piquada group. Indicated that the acupunture therapy may improve and sharpens the AD model big mouse's study memory ability(2) the model group MDA content obviously advance,the GSH-PX content obviously reduces,is quite even with the normal group has the unusual significance difference(P＜0.01).The sham-operation group MDA content,the GSH-PX active content and the normal group compare do not have the difference.The pre-piquada adds the moxibustion group MDA content,the GSH-PX active content and the model group comparison has the unusual significance difference(P＜0.01),the pre-Moxa group,the pre-piquada group MDA content,the GSH-PX active content and the model group comparison have the significance difference(P＜0.05).The pre-piquada adds the moxibustion group MDA content with the pre-Moxa group,the pre-piquada group comparison to have the unusual significance difference(P＜0.01),the pre-piquada adds the moxibustion group GSH-PX active content with the pre-Noxa group,the pre-piquada group comparison to have the significance difference(P＜0.05).The experimental result indicated that the pre-Moxa group,the pre-piquada group and the pre-piquada add the moxibustion group to be able the varying degree to reduce in the cerebrum seahorse the MDA content, raises the GSH-PX active content,especially the pre-piquada adds the moxibustion group effect to be best.ConclusionThis topic injected Aβ25-35 the success in the ventricles of the brain to duplicate the senile dementia animal model.The profit kidney accent supervises the law to be possible to eliminate the free radical,enhances organism oxidation resistance ability,reduces in the brain the free radical to neuron's damage,improves the AD model big mouse's study memory ability,thus plays delays the role which the brain gets older. "the profit kidney accent supervises" is embarks from the whole adjustment,prevents and controls AD for acupuncture the application to provide the experiment on clinical to rest on.
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