Chinese Herbal Compound Guanxinkang As Mechanism Of Lox-1 And Nf-kb In Rabbits

Objective To examine the levels of plasma homocysteine and the expression of NF-κB in aorta from methionine -fed rabbits and the effects of Guanxinkang on atherosclerotic lesion and NF-κB.T0 investigate the antiatherogenic effect of Guanxinkang .Methods Twenty-four rabbits were randomly divided into three groups. The control group were fed on common food 100g a day,the Hcy model were fed on high-methionine food which composed of common food containing 1% methionine 100g a day,while the Guanxinkang group with high-methionine food 100g/d+Guanxinkang powder 1.45g/kg/d. Blood samples were collected before (time 0) and after 4 and 8 weeks of experimental diets for measurement of serum triglycerides, total cholesterol (TC), and LDL, HDL cholesterol (LDL-C, HDL-C), and plasma hcy. At the end of 8^th weeks, the animals were put to death to establish pathological model of aorta for light microscopy to observe the vascular atherosclerotic lesion and its degree. The NF-κB expression in aortas was using ABC (Avidin-Biotin Complex) and DAB area ratio of the positive expressing cells of NF-κB (NF-κB%)was analyzed with Image-Pro Plus Image Analysis System and The relative expressing level of the mRNA expression of NF-κB was defined by optical density ratio of NF-κB to GAPDH respectively (NF-κB/GAPDH) by RT-PCR.Results(1) The level of plasma Hcy and serum TC, TG, LDL-C, and the ratios LDL-C/HDL-C and TC/HDL-C in the model group significantly higher than the control group(p<0.05), and markedly expression of NF-κB mRNA in aorta can be detectived by Immunohistochemistry. The endothelia cell hyperlasia on intima can be observed ,tunica media was infiltrated by the dot and local lipid . (2) Guanxinkang remarkably decreases the lipid in serum and plasma Hcy , inhibits NF-κB mRNA expression in aorta(P<0.05),also can attenuate aortic intimal proliferation.Conclusions:(1)The model of hyperhomocysteinemia(HHcy) can be established after high-methionine diet for 8 week. The HHcy can induce atherosclerosis in rabbits. (2) Guanxinkang markedly decreases the lipid in serum and HHcy, also inhibits NF-κB expression. Guanxinkang can prevent and theapy the HHcy, diminish the artery damage.