| AimTo investigate the possible mechanisms of YC in preventing and treating Hhcy and AS through observing the plasma Hcy level, changes of plasma ET, P-selectin and AT-Ⅲconcentration, and detecting the variation of serum NO, NOS and folacin content.Methods1. Experimental animals:2～3 months old male New Zealand White rabbits weighing 2.0-2.5kg.2. Model:Hhcy and AS rabbit model was induced by feeding high methionine diet (which include 2% methionine) for 5 months after the balloon injury in abdominal aorta.3. Animals grouping:56 male New Zealand White rabbits were randomly divided into 7 groups (n=8):normal group, model group, preventive group, low dose YC group, middle dose YC group, high dose YC group and western medicine group (folic acid, VitB12, VitB6).4. Detection contents:(1) Measurement of the plasma Hcy concentration by high performance liquid chromatography; (2) The serum NOS and NO content were detected by the method of nitric acid reductase; (3) Measurement of the serum folacin and the plasma ET concentration by RIA; (4) The plasma P-selectin and AT-Ⅲ concentration were detected by ELISA.Results1. Comparison of plasma Hcy concentrationThe plasma Hcy concentration in the model group was significantly higher than that in the normal group (P<0.01); After treatment with YC and western medicine, the Hcy concentration in each group was decreased in a different degree compared with the model group, and among those treatment groups, the preventive group had the lowest level of Hcy in plasma (P<0.01). The Hcy concentration in the preventive group, the high dose YC group was lower than that in the western medicine group (P<0.05); No significant difference was shown in the low dose YC group and middle dose YC group compared with the western medicine group (P>0.05).2. Comparison of serum NOS and NO contentThe serum NOS and NO content in the model group was significantly lower than that in the normal group (P<0.01); After treatment with YC and western medicine, the. NOS and NO content in each group was increased in a different degree compared with the model group, and among those treatment groups, the preventive group and the high dose YC group had the highest level of NOS and NO in serum (P<0.01). The NOS and NO content in the preventive group, the high dose YC group was higher than that in the western medicine group (P<0.01); There also had significant difference in the low dose YC group and middle dose YC group compared with the western medicine group (P<0.05).3. Comparison of serum folacin and plasma ET concentrationThe serum folacin concentration in the model group was significantly lower than that in the normal group (P<0.01); After treatment with YC and western medicine, the folacin concentration in each group was increased in a different degree compared with the model group(P<0.01). No significant difference was shown between every treatment group.The plasma ET concentration in the model group was significantly higher than that in the normal group (P<0.01); After treatment with YC and western medicine, the ET concentration in each group was decreased in a different degree compared with the model group, and among those treatment groups, the preventive group had the lowest level of ET in plasma. The ET concentration in the preventive group, the high dose YC group was lower than that in the western medicine group (P<0.01); No significant difference was shown in the low dose YC group and middle dose YC group compared with the western medicine group (P>0.05).4. Comparison of plasma P-selectin and AT-ⅢconcentrationThe plasma P-selectin concentration in the model group was significantly higher than that in the normal group (P<0.01); After treatment with YC and western medicine, the P-selectin concentration in each group was decreased in a different degree compared with the model group, and among those treatment groups, the high dose YC group had the lowest level of P-selectin in plasma. The P-selectin concentration in the preventive group, the high dose YC group was lower than that in the western medicine group (P<0.01); No significant difference was shown in the low dose YC group and middle dose YC group compared with the western medicine group (P>0.05).The plasma AT-Ⅲconcentration in the model group was significantly lower than that in the normal group (P<0.01); After treatment with YC and western medicine, the AT-Ⅲconcentration in each group was increased in a different degree compared with the model group(P<0.01); and among those treatment groups, the preventive group had the lowest level of AT-Ⅲin plasma. The AT-Ⅲconcentration in the preventive group, the high dose YC group was lower than that in the western medicine group (P<0.01).ConClusion1. YC could decrease the plasma Hcy concentration.2. An anti-atherosclerosis effect of YC was observed in our present study. YC could decrease the plasma ET concentration, increase serum NOS and NO content. The mechanisms were possibly associated with the protective effect of YC on vas endothelium.3. An inhibitory effect of YC on the inflammation in Hhcy rabbits was observed in our present study. YC could decrease the plasma P-selectin concentration, increase AT-Ⅲconcentration. The mechanisms were possibly associated with the suppressive effect of YC on platelet activation and the balance adjustability effect on blood solidification system.
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